Canola Hybrid Variety 9CN0089

ABSTRACT

The invention relates to a Canola hybrid variety designated 9CN0089, essentially derived variants of that Canola hybrid variety, to the cells, seeds, plants, and plant parts of this Canola hybrid variety 9CN0089. The invention also relates to methods for producing a canola plant containing in its genetic material one or more traits introgressed into 9CN0089 through backcross conversion and/or transformation, and to the Canola seed, plant and plant part produced thereby. The invention also relates to uses of 9CN0089.

FIELD

The present invention relates to the field of Brassica napus breeding(i.e. Canola breeding) and, more specifically, to the development of anew Canola hybrid variety, also referred to as “9CN0089”. Canola hybridvariety 9CN0089 was deposited with the National Collection ofIndustrial, Food and Marine Bacteria (NCIMB) on [XXXXXX] and was grantedthe Designation [XXXXXX].

BACKGROUND

The goal of oilseed rape breeding, in particular Canola breeding is tocombine various desirable traits in a single hybrid variety. Theresulting hybrid variety is of high quality and possesses a relativelylow level of erucic acid in the oil component and a relatively low levelof glucosinolates in the meal component, so it can be termed “Canola” inaccordance with the nomenclature used in plant science. Other desirabletraits may include stable and high yield, resistance to pests orphytopathogenic microorganisms, tolerance to heat and drought, reducingtime to crop maturity, reduction in pod shatter (shatter resistance) andpod drop, better agronomic quality, for example uniformity of plantcharacteristics as germination and stand establishment, growth rate,maturity, plant height, higher nutritional value, and growth rate.Canola is economically important due to the high-quality vegetable oilproduced from the harvested Canola seeds; therefore also an increasedoil content may be of interest as a breeding goal. While breedingefforts to date have provided a number of useful Canola lines and hybridvarieties with beneficial traits, there remains a great need in the artfor new Canola hybrid varieties and lines with further improved traitsregarding their agronomic characteristics. Such plants would benefitfarmers and consumers alike by improving overall crop yields and/orquality.

SUMMARY

In one aspect of the invention, a new Canola hybrid variety designated9CN0089 is provided. The invention also relates to the seeds of the9CN0089 Canola hybrid variety, wherein a representative sample of saidseed has been deposited under Accession Number [XXXXXX], to plants ofthe 9CN0089 Canola hybrid variety, and to methods for producing a Canolaplant by crossing the 9CN0089 Canola hybrid variety with itself oranother Canola plant or Canola line (whether by use of male sterility oropen pollination), and to methods for producing a Canola plantcontaining in its genetic material one or more transgenes, and totransgenic plants produced by that method. This invention also relatesto Canola seeds and plants produced by crossing the 9CN0089 Canolahybrid variety with another line. In another aspect the inventionprovides for a hybrid variety of Canola designated 9CN0089. Theinvention also provides for a plurality of seeds of the new hybridvariety, plants produced from growing the seeds of the new variety9CN0089, and progeny of any of these. Especially, progeny retaining oneor more (or all) of the “distinguishing characteristics” or one or more(or all) of the “essential morphological and physiologicalcharacteristics” or essentially all physiological and morphologicalcharacteristics of 9CN0089 referred to herein, are encompassed herein aswell as methods for producing these. In one aspect, such progeny have(essentially) all the physiological and morphological characteristics ofCanola hybrid variety 9CN0089 when grown under the same environmentalconditions. Further, Canola seeds produced on a plant grown from theseseeds is provided. In another embodiment a cell of a Canola plant isprovided which is produced by crossing Canola plants and harvesting theresultant seed, wherein at least one Canola plant is Canola hybridvariety 9CN0089 wherein representative seed of said variety has beendeposited under the Accession Number [XXXXXX]. In another embodiment acell of a Canola plant is provided wherein the cell is of an F1 hybridCanola seed, wherein a plant produced from said seed has essentially thephysiological and morphological characteristics of a plant of Canolahybrid variety 9CN0089 when grown in the same environmental conditions.In another embodiment a cell of a progeny Canola variety derived fromCanola hybrid variety 9CN0089 is provided, comprising a desired trait,said progeny Canola variety produced by a method comprising the stepsof:

-   (a) crossing a Canola hybrid variety 9CN0089 plant with a plant of    another Canola variety that comprises a desired trait to produce F1    progeny plants;-   (b) selecting one or more F1 progeny plants that have the desired    trait to produce selected progeny plants;-   (c) crossing the selected progeny plants with a Canola hybrid    variety 9CN0089 plant to produce backcross progeny plants;-   (d) selecting for backcross progeny plants that have the desired    trait and physiological and morphological characteristics of the    Canola hybrid variety 9CN0089 plant to produce selected backcross    progeny plants; and-   (e) repeating steps (c) and (d) a sufficient number of times in    succession to produce selected second or higher backcross progeny    plants that comprise the desired trait and essentially all of the    physiological and morphological characteristics of Canola hybrid    variety 9CN0089 when grown in the same environmental conditions. In    yet another embodiment of the invention, an Essentially Derived    Variety of 9CN0089 having one, two or three physiological and/or    morphological characteristics which are different from those of    9CN0089 and which otherwise has all the physiological and    morphological characteristics of 9CN0089, wherein a representative    sample of seed of variety 9CN0089 has been deposited under Accession    Number [XXXXXX] is provided. Also a plant part derived from Canola    hybrid variety 9CN0089 is provided, wherein said plant part is    selected from the group consisting of: harvested fruits or parts    thereof, pollen, ovules, cells, leaves or parts thereof, petioles,    shoots or parts thereof, stems or parts thereof, roots or parts    thereof, cuttings, seeds, hypocotyl, cotyledon, flowers or parts    thereof. In another aspect a method for the protection of a group of    cultivated plants of Canola hybrid variety 9CN0089 in a field is    provided wherein harmful microorganisms, pests or weeds are    controlled by the application of a composition comprising one or    more microbicidal, insecticidal or herbicidal active ingredients.

In particular embodiments, there is provided:

1. A plant cell of a Canola hybrid variety designated 9CN0089, wherein arepresentative sample of seed of that variety has been deposited underthe Accession Number [XXXXXX].

2. The plant cell of paragraph 1 wherein the plant cell is a seed cell.

3. The plant cell from a descendant of the Canola plant as defined inparagraph 1 limited to the first, second, third, fourth, fifth, sixth,seventh, eighth, ninth, or tenth generation.

4. The plant cell according to paragraph 3, wherein said descendant hasessentially the physiological and morphological characteristics of aplant of Canola hybrid variety 9CN0089 when grown in the sameenvironmental conditions.

5. A cell of a Canola plant produced by crossing Canola plants andharvesting the resultant seed, wherein at least one Canola plant isCanola hybrid variety 9CN0089 wherein representative seed of saidvariety has been deposited under the Accession Number [XXXXXX].

6. The cell of paragraph 5, wherein the cell is of an F1 hybrid Canolaseed, wherein a plant produced from said seed has essentially thephysiological and morphological characteristics of a plant of Canolahybrid variety 9CN0089 when grown in the same environmental conditions.

7. A cell of a progeny Canola variety derived from Canola hybrid variety9CN0089, comprising a desired trait, said progeny Canola varietyproduced by a method comprising the steps of:

-   (a) crossing a Canola hybrid variety 9CN0089 plant as defined in    paragraph 1 or 2 with a plant of another Canola variety that    comprises a desired trait to produce F1 progeny plants;-   (b) selecting one or more F1 progeny plants that have the desired    trait to produce selected progeny plants;-   (c) crossing the selected progeny plants with a Canola hybrid    variety 9CN0089 plant to produce backcross progeny plants;-   (d) selecting for backcross progeny plants that have the desired    trait and physiological and morphological characteristics of the    Canola hybrid variety 9CN0089 plant to produce selected backcross    progeny plants; and-   (e) repeating steps (c) and (d) a sufficient number of times in    succession to produce selected second or higher backcross progeny    plants that comprise the desired trait and essentially all of the    physiological and morphological characteristics of Canola hybrid    variety 9CN0089 when grown in the same environmental conditions.

8. A locus converted plant cell of a locus converted plant obtained byintroducing a locus conversion into Canola hybrid variety 9CN0089wherein representative seed of said variety has been deposited under theAccession Number [XXXXXX], and wherein the locus converted plant cell isidentical to a cell from variety 9CN0089 except for the locus conversionand the locus converted plant expresses essentially the physiologicaland morphological characteristics of Canola hybrid variety 9CN0089.

9. The plant cell of paragraph 8, wherein the locus conversion confers atrait and the trait is: male sterility, site-specific recombination,abiotic stress tolerance, altered phosphate, altered antioxidants,altered fatty acids, altered essential amino acids, alteredcarbohydrates, improved shatter resistance, herbicide resistance, insectresistance or disease resistance.

10. A plant cell of an Essentially Derived Variety of 9CN0089 havingone, two or three physiological and/or morphological characteristicswhich are different from those of 9CN0089 and which otherwise has allthe physiological and morphological characteristics of 9CN0089, whereina representative sample of seed of variety 9CN0089 has been depositedunder the Accession Number [XXXXXX].

11. Use of a Canola plant of Canola hybrid variety 9CN0089,representative seed of said variety having been deposited under theAccession Number [XXXXXX] to breed a second plant.

12. Use of a Canola plant of Canola hybrid variety 9CN0089,representative seed of said variety having been deposited under theAccession Number [XXXXXX] to breed an inbred.

13. The use of paragraph 12, wherein the Canola plant is used to producea haploid that is subsequently doubled to produce a double haploidinbred.

14. Use of a Canola seed of Canola hybrid variety 9CN0089,representative seed of said variety having been deposited under theAccession Number [XXXXXX] to produce clean seed.

15. A Canola seed cell of Canola hybrid variety 9CN0089, representativeseed of said variety having been deposited under the Accession Number[XXXXXX], wherein the seed is clean seed.

16. A Canola seed cell of Canola hybrid variety 9CN0089, representativeseed of said variety having been deposited under the Accession Number[XXXXXX], wherein the seed is treated.

17. Use of a Canola seed of Canola hybrid variety 9CN0089,representative seed of said variety having been deposited under theAccession Number [XXXXXX] to produce treated seed.

18. The use according to paragraph 16 or 17 wherein the seed is treatedwith fungicide or pesticide.

19. Use of a Canola seed of Canola hybrid variety 9CN0089,representative seed of said variety having been deposited under theAccession Number [XXXXXX] to grow subsequent generations.

20. Use of a collection of seed from a commercial bag of Canola hybridvariety 9CN0089, representative seed of said variety having beendeposited under the Accession Number [XXXXXX], to grow a commercialcrop.

21. Use of a Canola hybrid plant designated 9CN0089, seed of said hybridhaving been deposited under the Accession Number [XXXXXX], to produce F2seed.

22. Use of an F1 hybrid Canola plant designated 9CN0089, seed of saidhybrid having been deposited under the Accession Number [XXXXXX] toproduce a commodity product comprising seed oil, meal, fiber, orprotein.

23. The use of paragraph 22, wherein the commodity product comprisesseed oil.

24. Use of a Canola hybrid plant designated 9CN0089, seed of said hybridhaving been deposited under the Accession Number [XXXXXX], to producecrushed non-viable F2 seed.

25. The use of paragraph 24 wherein the crushed non-viable F2 seed isfor use in the production of seed oil, meal, fiber, or protein.

26. Use of a Canola seed of Canola hybrid variety 9CN0089,representative seed of said variety having been deposited under theAccession Number [XXXXXX] as a recipient of a locus conversion.

27. Use of a Canola seed of Canola hybrid variety 9CN0089,representative seed of said variety having been deposited under theAccession Number [XXXXXX] to grow a commercial crop.

28. A method for the protection of a group of cultivated plants ofCanola hybrid variety 9CN0089 representative seed of said variety havingbeen deposited under the Accession Number [XXXXXX] in a field whereinweeds are controlled by the application of a composition comprising oneor more herbicidal active ingredients.

29. The method according to paragraph 28 wherein one or more herbicideis selected from the group comprising amitrol, carfentrazone, clethodim,clopyralid, dicamba, diquat, ethalfluralin, ethametsulfuron-methyl,florasulam, imazamox, imazapyr, glufosinate, glufosinate-ammonium,glyphosate, MCPA amine, MCPA ester, metsulfuron, quizalofop-p-ethyl,quinclorac, saflufenacil, triallate, and trifluralin.

30. The method according to paragraph 28 or 29 wherein the herbicide isglufosinate or glufosinate ammonium.

31. The method according to paragraph 30 wherein glufosinate orglufosinate ammonium is applied in mixture or in sequence with one ormore herbicides selected from the group comprising amitrol,carfentrazone, clethodim, clopyralid, dicamba, diquat, ethalfluralin,ethametsulfuron-methyl, florasulam, imazamox, imazapyr, glyphosate, MCPAamine, MCPA ester, metsulfuron, quizalofop-p-ethyl, quinclorac,saflufenacil, triallate, and trifluralin.

32. A method for the protection of a group of cultivated plants ofCanola hybrid variety 9CN0089 representative seed of said variety havingbeen deposited under the Accession Number [XXXXXX] in a field whereinharmful microorganisms are controlled by the application of acomposition comprising one or more fungicidal active ingredients.

33. The method according to paragraph 32 wherein one or more herbicideis selected from the group comprising azoxystrobin, benzovindiflupyr,boscalid, cyprodinil, fludioxonil, fluxapyroxad, fluopyram,ipfentrifluconazole, iprodione, isoflucypram, metalaxyl, mefenoxam,mefentrifluconazole, metconazole, penthiopyrad, picoxystrobin,propiconazole, prothioconazole, pyraclostrobin, pyraziflumid,pydiflumetofen, sedaxane, and tebuconazole.

34. A method for the protection of a group of cultivated plants ofCanola hybrid variety 9CN0089 representative seed of said variety havingbeen deposited under the Accession Number [XXXXXX] in a field whereinpests are controlled by the application of a composition comprising oneor more insecticidal active ingredients.

35. The method according to paragraph 34 wherein the one or moreinsecticidal active ingredient is selected from the group comprisingbroflanilide, carbaryl, carbofuran, chlorantraniliprole, chlorpyrifos,cypermethrin, cyclaniliprole, cyhalodiamide, clothianidin, deltamethrin,dimethoate, cyantraniliprole, cyhalothrin-lambda, imidacloprid,lambda-cyhalothrin, permethrin, sulfoxaflor, spirotetramate,tetraniliprole, and thiamethoxam.

36. A method for the protection of a group of cultivated plants ofCanola hybrid variety 9CN0089 representative seed of said variety havingbeen deposited under the Accession Number [XXXXXX] in a field whereinharmful microorganisms and/or pests are controlled by the application ofa composition comprising one or more fungicidal or insecticidal activeingredients onto the seeds of said variety before seeding.

37. The method according to paragraph 36 wherein one or more fungicidalor insecticidal active ingredient is selected from the group comprisingbroflanilide, carbaryl, carbofuran, chlorantraniliprole, chlorpyrifos,cypermethrin, cyclaniliprole, cyhalodiamide, clothianidin, deltamethrin,dimethoate, cyantraniliprole, cyhalothrin-lambda, imidacloprid,lambda-cyhalothrin, permethrin, sulfoxaflor, spirotetramate,tetraniliprole, thiamethoxam, azoxystrobin, benzovindiflupyr, boscalid,cyprodinil, fludioxonil, fluxapyroxad, fluopyram, ipfentrifluconazole,iprodione, isoflucypram, metalaxyl, mefenoxam, mefentrifluconazole,metconazole, penthiopyrad, picoxystrobin, propiconazole,prothioconazole, pyraclostrobin, pyraziflumid, pydiflumetofen, sedaxane,and tebuconazole.

38. A plant or part thereof of a Canola hybrid variety designated9CN0089, wherein a representative sample of seed of that variety hasbeen deposited under the Accession Number [XXXXXX].

39. The plant part of paragraph 38, wherein said plant part is selectedfrom the group consisting of: harvested fruits or parts thereof, pollen,ovules, cells, leaves or parts thereof, petioles, shoots or partsthereof, stems or parts thereof, roots or parts thereof, cuttings,seeds, hypocotyl, cotyledon, and flowers or parts thereof.

40. A seed of the plant of paragraph 38.

41. A descendant plant or part thereof of the Canola plant as defined inparagraph 38 or the seed of paragraph 40 limited to the first, second,third, fourth, fifth, sixth, seventh, eighth, ninth, or tenthgeneration.

42. The plant part of paragraph 41, wherein said plant part is selectedfrom the group consisting of: harvested fruits or parts thereof, pollen,ovules, cells, leaves or parts thereof, petioles, shoots or partsthereof, stems or parts thereof, roots or parts thereof, cuttings,seeds, hypocotyl, cotyledon, and flowers or parts thereof.

43. A descendant plant according to paragraph 41, wherein saiddescendant has essentially the physiological and morphologicalcharacteristics of a plant of Canola hybrid variety 9CN0089 when grownin the same environmental conditions.

44. A Canola plant produced by crossing Canola plants and harvesting theresultant seed, wherein at least one Canola plant is Canola hybridvariety 9CN0089 wherein representative seed of said variety has beendeposited under the Accession Number [XXXXXX].

45. The plant of paragraph 44, wherein the plant is an F1 hybrid Canolaplant that has essentially the physiological and morphologicalcharacteristics of a plant of Canola hybrid variety 9CN0089 when grownin the same environmental conditions.

46. A plant of a progeny Canola variety derived from Canola hybridvariety 9CN0089, comprising a desired trait, said progeny Canola varietyproduced by a method comprising the steps of:

-   (a) crossing a Canola hybrid variety 9CN0089 plant as defined in    paragraph 38 with a plant of another Canola variety that comprises a    desired trait to produce F1 progeny plants;-   (b) selecting one or more F1 progeny plants that have the desired    trait to produce selected progeny plants;-   (c) crossing the selected progeny plants with a Canola hybrid    variety 9CN0089 plant to produce backcross progeny plants;-   (d) selecting for backcross progeny plants that have the desired    trait and physiological and morphological characteristics of the    Canola hybrid variety 9CN0089 plant to produce selected backcross    progeny plants; and-   (e) repeating steps (c) and (d) a sufficient number of times in    succession to produce selected second or higher backcross progeny    plants that comprise the desired trait and essentially all of the    physiological and morphological characteristics of Canola hybrid    variety 9CN0089 when grown in the same environmental conditions.

47. A locus converted plant obtained by introducing a locus conversioninto Canola hybrid variety 9CN0089 wherein representative seed of saidvariety has been deposited under the Accession Number [XXXXXX], andwherein the locus converted plant is identical to the variety 9CN0089except for the locus conversion and the locus converted plant expressesessentially the physiological and morphological characteristics ofCanola hybrid variety 9CN0089.

48. The plant of paragraph 47, wherein the locus conversion confers atrait and the trait is: male sterility, site-specific recombination,abiotic stress tolerance, altered phosphate, altered antioxidants,altered fatty acids, altered essential amino acids, alteredcarbohydrates, improved shatter resistance, herbicide resistance, insectresistance or disease resistance.

49. A plant of an Essentially Derived Variety of 9CN0089 having one, twoor three physiological and/or morphological characteristics which aredifferent from those of 9CN0089 and which otherwise has all thephysiological and morphological characteristics of 9CN0089, wherein arepresentative sample of seed of variety 9CN0089 has been depositedunder the Accession Number [XXXXXX].

50. A method of producing a second plant, the method comprising selfinga Canola plant of Canola hybrid variety 9CN0089, representative seed ofsaid variety having been deposited under the Accession Number [XXXXXX],or breeding a Canola plant of Canola hybrid variety 9CN0089 with anotherplant, and growing the resulting seed.

51. A second plant, or part thereof, produced by the method of paragraph50.

52. A seed of a second plant produced by the method of paragraph 50.

53. A method of producing an inbred plant, the method comprisingselecting a plant and selfing the selected plant and its descendants forseveral generations to produce the inbred plant, wherein the selectedplant is derived from a Canola plant of Canola hybrid variety 9CN0089,representative seed of said variety having been deposited under theAccession Number [XXXXXX].

54. The method of paragraph 53 further comprising doubling a haploid toproduce a double haploid inbred plant, wherein the haploid is theselected plant or descendant thereof derived from the Canola plant ofCanola hybrid variety 9CN0089.

55. An inbred plant, or part thereof, produced by the method ofparagraph 53 or 54.

56. A seed of an inbred plant produced by the method of paragraph 53 or54.

57. A method of producing a clean seed, the method comprising the stepsof obtaining a seed of Canola hybrid variety 9CN0089, representativeseed of said variety having been deposited under the Accession Number[XXXXXX], and cleaning said seed.

58. A clean seed produced by the method of paragraph 57.

59. A Canola seed of Canola hybrid variety 9CN0089, representative seedof said variety having been deposited under the Accession Number[XXXXXX], wherein the seed is clean seed.

60. A Canola seed of Canola hybrid variety 9CN0089, representative seedof said variety having been deposited under the Accession Number[XXXXXX], wherein the seed is treated.

61. The Canola seed according to paragraph 60, wherein the seed istreated with fungicide or pesticide.

62. A method of producing a treated seed, the method comprising thesteps of obtaining a Canola seed of Canola hybrid variety 9CN0089,representative seed of said variety having been deposited under theAccession Number [XXXXXX], and treating said seed.

63. The method of paragraph 62, wherein the treating step comprisestreating with fungicide or pesticide.

64. A treated seed produced by the method of paragraph 62 or 63.

65. A method of producing a subsequent generation, the method comprisinggrowing a Canola seed of Canola hybrid variety 9CN0089, representativeseed of said variety having been deposited under the Accession Number[XXXXXX], or a seed of a descendant thereof, to generate a plant, andselfing or breeding said plant to produce seed, and growing the seed.

66. A plant of a subsequent generation produced by the method ofparagraph 65.

67. A seed of a plant of a subsequent generation produced by the methodof paragraph 65.

68. A method of producing a commercial crop, the method comprisinggrowing a collection of seed from a commercial bag of Canola hybridvariety 9CN0089, representative seed of said variety having beendeposited under the Accession Number [XXXXXX].

69. A commercial crop produced by the method of paragraph 68.

70. A method of producing F2 seed, the method comprising selfing aCanola hybrid plant designated 9CN0089, seed of said hybrid having beendeposited under the Accession Number [XXXXXX], or breeding said plantwith another plant to produce F1 seed, growing said F1 seed to produceF1 plants, and selfing or breeding said F1 plants to produce F2 seed.

71. F2 seed produced by the method of paragraph 70.

72. A F2 plant grown from the F2 seed produced by the method ofparagraph 70.

73. A method of producing a commodity product, the method comprisingobtaining seed produced by an F1 hybrid Canola plant designated 9CN0089,seed of said hybrid having been deposited under the Accession Number[XXXXXX], and preparing the commodity product, wherein said commodityproduct comprises seed oil, meal, fiber, or protein.

74. The method of paragraph 73, wherein the commodity product comprisesseed oil.

75. A commodity product produced by the method of paragraph 73 or 74.

76. Seed oil produced by the method of paragraph 74.

77. A method of producing crushed non-viable F2 seed, the methodcomprising obtaining F2 seed produced by a Canola hybrid plantdesignated 9CN0089, seed of said hybrid having been deposited under theAccession Number [XXXXXX], and crushing the F2 seed.

78. Crushed non-viable F2 seed produced by the method of paragraph 77.

79. The method of paragraph 77 further comprising preparing seed oil,meal, fiber, or protein from the crushed non-viable F2 seed.

80. Seed oil, meal, fiber, or protein produced by the method ofparagraph 79.

81. A method of producing a locus converted plant, the method comprisingintroducing a locus conversion into a Canola seed of Canola hybridvariety 9CN0089 wherein representative seed of said variety has beendeposited under the Accession Number [XXXXXX], and wherein the locusconverted plant is identical to the variety 9CN0089 except for the locusconversion and the locus converted plant expresses essentially thephysiological and morphological characteristics of Canola hybrid variety9CN0089.

82. A locus converted plant, or a part thereof, produced by the methodof paragraph 81.

83. A seed of a locus converted plant produced by the method ofparagraph 81.

84. A method of producing a commercial crop, the method comprisingplanting Canola seed of Canola hybrid variety 9CN0089, representativeseed of said variety having been deposited under the Accession Number[XXXXXX], and growing the commercial crop.

85. A commercial crop produced by the method of paragraph 84.

86. A method of producing a progeny Canola variety derived from Canolahybrid variety 9CN0089, comprising a desired trait, said methodcomprising the steps of:

-   (a) crossing a Canola hybrid variety 9CN0089 plant as defined in    paragraph 38 with a plant of another Canola variety that comprises a    desired trait to produce F1 progeny plants;-   (b) selecting one or more F1 progeny plants that have the desired    trait to produce selected progeny plants;-   (c) crossing the selected progeny plants with a Canola hybrid    variety 9CN0089 plant to produce backcross progeny plants;-   (d) selecting for backcross progeny plants that have the desired    trait and physiological and morphological characteristics of the    Canola hybrid variety 9CN0089 plant to produce selected backcross    progeny plants; and-   (e) repeating steps (c) and (d) a sufficient number of times in    succession to produce selected second or higher backcross progeny    plants that comprise the desired trait and essentially all of the    physiological and morphological characteristics of Canola hybrid    variety 9CN0089 when grown in the same environmental conditions.

87. A plant of a progeny Canola variety, or part thereof, produced bythe method of paragraph 86.

88. A seed of a plant of a progeny Canola variety produced by the methodof paragraph 86.

89. A plant or part thereof of a Canola hybrid variety designated9CN0089, wherein a representative sample of seed of that variety hasbeen deposited under the Accession Number [XXXXXX], wherein the partthereof comprises at least one cell of Canola hybrid variety designated9CN0089.

90. A method for the protection of a group of cultivated plants of theplant of paragraph 89, comprising one or more of:

-   (a) applying a composition comprising one or more herbicidal active    ingredients, in a field wherein weeds are to be controlled;-   (b) applying a composition comprising one or more fungicidal active    ingredients, in a field wherein harmful microorganisms are to be    controlled; and/or-   (c) applying a composition comprising one or more insecticidal    active ingredients, in a field wherein pests are to be controlled.

91. The method according to paragraph 90, wherein:

-   (a) the one or more herbicidal active ingredients is amitrol,    carfentrazone, clethodim, clopyralid, dicamba, diquat,    ethalfluralin, ethametsulfuron-methyl, florasulam, imazamox,    imazapyr, glufosinate, glufosinate-ammonium, glyphosate, MCPA amine,    MCPA ester, metsulfuron, quizalofop-p-ethyl, quinclorac,    saflufenacil, triallate, and/or trifluralin;-   (b) the one or more fungicidal active ingredients is azoxystrobin,    benzovindiflupyr, boscalid, cyprodinil, fludioxonil, fluxapyroxad,    fluopyram, ipfentrifluconazole, iprodione, isoflucypram, metalaxyl,    mefenoxam, mefentrifluconazole, metconazole, penthiopyrad,    picoxystrobin, propiconazole, prothioconazole, pyraclostrobin,    pyraziflumid, pydiflumetofen, sedaxane, and/or tebuconazole; and/or-   (c) the one or more insecticidal active ingredients is broflanilide,    carbaryl, carbofuran, chlorantraniliprole, chlorpyrifos,    cypermethrin, cyclaniliprole, cyhalodiamide, clothianidin,    deltamethrin, dimethoate, cyantraniliprole, cyhalothrin-lambda,    imidacloprid, lambda-cyhalothrin, permethrin, sulfoxaflor,    spirotetramate, tetraniliprole, and/or thiamethoxam.

92. The method according to paragraph 91, wherein the one or moreherbicidal active ingredients comprises glufosinate or glufosinateammonium.

93. A method of producing an inbred plant, the method comprisingselecting a plant and selfing the selected plant and its descendants forseveral generations to produce the inbred plant, wherein the selectedplant is derived from the plant of paragraph 89.

94. The method of paragraph 93, further comprising doubling a haploid toproduce a double haploid inbred plant, wherein the haploid is theselected plant or descendant thereof derived from the Canola plant ofCanola hybrid variety 9CN0089.

95. A method of producing F2 seed, the method comprising selfing theCanola hybrid plant of paragraph 89, or breeding said plant with anotherplant to produce F1 seed, growing said F1 seed to produce F1 plants, andselfing or breeding said F1 plants to produce F2 seed.

96. A method of producing a clean seed, the method comprising obtainingthe seed of Canola hybrid variety 9CN0089, wherein a representativesample of seed of that variety has been deposited under Accession Number[XXXXXX], and cleaning said seed.

97. A treated seed of Canola hybrid variety 9CN0089, representative seedof said variety having been deposited under the Accession Number[XXXXXX].

98. A method of producing a treated seed of paragraph 97, the methodcomprising obtaining the seed of Canola hybrid variety 9CN0089, whereina representative sample of seed of that variety has been deposited underAccession Number [XXXXXX], and treating said seed.

99. A method of producing a commodity product, the method comprisingobtaining seed produced by an F1 hybrid Canola plant designated 9CN0089,seed of said hybrid having been deposited under the Accession Number[XXXXXX], and preparing the commodity product, wherein said commodityproduct comprises seed oil, meal, fiber, or protein.

100. A method of producing a commercial crop, the method comprisingplanting the seed of Canola hybrid variety 9CN0089, wherein arepresentative sample of seed of that variety has been deposited underthe Accession Number [XXXXXX], and growing the commercial crop.

DEFINITIONS

In the description and tables which follow, a number of terms are used.In order to aid in a clear and consistent understanding of thespecification, the following definitions and evaluation criteria areprovided.

“Canola” refers herein to seeds or plants of the genus Brassica(Brassica napus, Brassica rapa or Brassica juncea) from which the oilshall contain less than 2% erucic acid in its fatty acid profile and thesolid component shall contain less than 30 micromoles of any one or anymixture of 3-butenyl glucosinolate, 4-pentenyl glucosinolate,2-hydroxy-3 butenyl glucosinolate, and 2-hydroxy- 4-pentenylglucosinolate per gram of air-dry, oil-free solid.

The terms “Canola hybrid variety 9CN0089”, “9CN0089”, or “Canola hybridvariety designated 9CN0089”, “Canola variety 9CN0089” are usedinterchangeably herein and refer to a plant of Canola hybrid variety9CN0089, representative seed of which having been deposited underAccession Number [XXXXXX]. As used herein, the term “plant” includes thewhole plant or any parts such as plant organs, plant cells, plantprotoplasts, plant cell cultures or tissue cultures from which wholeplants can be regenerated, plant callus, plant cell clumps, planttransplants, seedlings, plant cells that are intact in plants, plantclones or micropropagations, or parts of plants (e.g., harvestedtissues, fruits or organs), such as plant cuttings, vegetativepropagations, embryos, pollen, ovules, flowers, leaves, fruits, fruitflesh, seeds, clonally propagated plants, roots, stems, stalks, roottips, grafts, parts of any of these and the like, or derivativesthereof, preferably having the same genetic make-up (or very similargenetic make-up) as the plant from which it is obtained. Also anydevelopmental stage is included, such as seedlings, cuttings prior orafter rooting, mature and/or immature plants or mature and/or immatureleaves.

“Tissue culture” refers to a composition comprising isolated cells ofthe same or a different type or a collection of such cells organizedinto parts of a plant. Tissue culture of various tissues of Canola andregeneration of plants therefrom is well known and widely published(see, e.g., Sang-Gu et al. (1988), Plant Cell, Tissue and Organ Culture12: 67-74; Colijn-Hooymans (1994), Plant Cell, Tissue and Organ Culture39: 211-217). Similarly, the skilled person is well-aware how to preparea “cell culture”.

A plant having “(essentially) all the physiological and morphologicalcharacteristics” means a plant having the physiological andmorphological characteristics when grown under the same environmentalconditions of the plant from which it was derived, e.g. the progenitorplant, the parent, the recurrent parent, the plant used for tissue- orcell culture, etc. In certain embodiments the plant has all thephysiological and morphological characteristics, except for certaincharacteristics mentioned, e.g. the characteristic(s) derived from aconverted or introduced gene or trait and/or except for thecharacteristics which differ in an EDV.

A plant having one or more “essential physiological and/or morphologicalcharacteristics” or one or more “distinguishing characteristics” refersto a plant having (or retaining) one or more of the characteristicsmentioned in Table 1 when grown under the same environmental conditionsthat distinguish 9CN0089 from the most similar varieties, such as butnot limited to oil content, protein content, yield, time to maturity,disease resistance, standability, lodging or shatter resistance. Thephysiological and/or morphological characteristics mentioned above arecommonly evaluated at significance levels of 1%, 5% or 10% significancelevel, when measured under the same environmental conditions. Forexample, a progeny plant of Canola hybrid variety 9CN0089 may have oneor more (or all) of the essential physiological and/or morphologicalcharacteristics of Canola hybrid variety 9CN0089 listed in Table 1, asdetermined at the 5% significance level when grown under the sameenvironmental conditions. As used herein, the term “variety” or“cultivar” means a plant grouping within a single botanical taxon of thelowest known rank, which grouping, irrespective of whether theconditions for the grant of a breeder’s right are fully met, can bedefined by the expression of the characteristics resulting from a givengenotype or combination of genotypes, distinguished from any other plantgrouping by the expression of at least one of the said characteristicsand considered as a unit with regard to its suitability for beingpropagated unchanged.

A variety is referred to as an “Essentially Derived Variety” (EDV) i.e.,shall be deemed to be essentially derived from another variety, “theinitial variety” when (i) it is predominantly derived from the initialvariety, or from a variety that is itself predominantly derived from theinitial variety, while retaining the expression of the essentialcharacteristics that result from the genotype or combination ofgenotypes of the initial variety; (ii) it is clearly distinguishablefrom the initial variety; and (iii) except for the differences whichresult from the act of derivation, it conforms to the initial variety inthe expression of the essential characteristics that result from thegenotype or combination of genotypes of the initial variety. Thus, anEDV may be obtained for example by the selection of a natural or inducedmutant, or of a somaclonal variant, the selection of a variantindividual from plants of the initial variety, backcrossing, ortransformation by genetic engineering.

“Plant line” is for example a breeding line which can be used to developone or more varieties. “Hybrid variety” or “F1 hybrid” refers to theseeds harvested from crossing two inbred (nearly homozygous) parentallines. For example, the female parent is pollinated with pollen of themale parent to produce hybrid (F1) seeds on the female parent.

“Regeneration” refers to the development of a plant from cell culture ortissue culture or vegetative propagation.

“Selfing” refers to self-pollination of a plant, i.e., the transfer ofpollen from the anther to the stigma of the same plant.

“Crossing” refers to the mating of two parent plants.

“Average” refers herein to the arithmetic mean. “Substantiallyequivalent” refers to a characteristic that, when compared, does notshow a statistically significant difference (e.g., p = 0.05) from themean.

“Locus” (plural loci) refers to the specific location of a gene or DNAsequence on a chromosome. A locus may confer a specific trait.

“Allele” refers to one or more alternative forms of a gene locus. All ofthese loci relate to one trait. Sometimes, different alleles can resultin different observable phenotypic traits, such as differentpigmentation. However, many variations at the genetic level result inlittle or no observable variation. If a multicellular organism has twosets of chromosomes, i.e. diploid, these chromosomes are referred to ashomologous chromosomes. Diploid organisms have one copy of each gene(and therefore one allele) on each chromosome. If both alleles are thesame, they are homozygotes. If the alleles are different, they areheterozygotes.

“Genotype” refers to the genetic composition of a cell or organism.

The term “traditional breeding techniques” encompasses herein crossing,selfing, selection, double haploid production, embryo rescue, protoplastfusion, marker assisted selection, mutation breeding etc. as known tothe breeder (i.e. methods other than geneticmodification/transformation/transgenic methods), by which, for example,a genetically heritable trait can be transferred from one Canola line orvariety to another.

“Backcrossing” is a traditional breeding technique used to introduce atrait into a plant line or variety. The plant containing the trait iscalled the donor plant and the plant into which the trait is transferredis called the recurrent parent. An initial cross is made between thedonor parent and the recurrent parent to produce progeny plants. Progenyplants which have the trait are then crossed to the recurrent parent.After several generations of backcrossing and/or selfing the recurrentparent comprises the trait of the donor. The plant generated in this waymay be referred to as a “single trait converted plant”.

“Progeny” as used herein refers to plants derived from a plant of aCanola hybrid variety 9CN0089. Progeny may be derived by regeneration ofcell culture or tissue culture or parts of a plant of Canola hybridvariety 9CN0089 or selfing of a plant designated 9CN0089 or by producingseeds of a plant of Canola hybrid variety 9CN0089. In furtherembodiments, progeny may also encompass plants derived from crossing ofat least one plant of Canola hybrid variety 9CN0089 with another Canolaplant of the same or another variety or (breeding) line, or wild plantsof Brassica species, backcrossing, inserting of a locus into a plant ormutation. A progeny is, e.g., a first-generation progeny, i.e. theprogeny is directly derived from, obtained from, obtainable from orderivable from the parent plant by, e.g., traditional breeding methods(selfing and/or crossing) or regeneration. However, the term “progeny”generally encompasses further generations such as second, third, fourth,fifth, sixth, seventh or more generations, i.e., generations of plantswhich are derived from, obtained from, obtainable from or derivable fromthe former generation by, e.g., traditional breeding methods,regeneration or genetic transformation techniques. For example, a secondgeneration progeny can be produced from a first generation progeny byany of the methods mentioned above.

The terms “gene converted” or “conversion plant” in this context referto Canola plants which are developed by backcrossing wherein essentiallyall of the desired morphological and physiological characteristics ofparent are recovered in addition to the one or more genes transferredinto the parent via the backcrossing technique or via geneticengineering. Likewise a “Single Locus Converted (Conversion) Plant”refers to plants which are developed by plant breeding techniquescomprising or consisting of backcrossing, wherein essentially all of thedesired morphological and physiological characteristics of a Canolavariety are recovered in addition to the characteristics of the singlelocus having been transferred into the variety via the backcrossingtechnique and/or by genetic transformation.

“Transgene” or “chimeric gene” refers to a genetic locus comprising aDNA sequence which has been introduced into the genome of a Canola plantby transformation. A plant comprising a transgene stably integrated intoits genome is referred to as “transgenic plant”.

The term “mean” refers to the arithmetic mean of several measurements.The skilled person understands that the appearance of a plant depends tosome extent on the growing conditions of said plant. Thus, the skilledperson will know typical growing conditions for Canola plants describedherein. The mean, if not indicated otherwise within this application,refers to the arithmetic mean of measurements on at least different,randomly selected plants of a variety or line.

The term “Anther Fertility” means the ability of a plant to producepollen; measured by pollen production. 1 = sterile, 9 = all anthersshedding pollen (vs. Pollen Formation which is amount of pollenproduced).

The term “Anther Arrangement” means the general disposition of theanthers in typical fully opened flowers is observed.

The term “Chlorophyll Content” means the typical chlorophyll content ofthe mature seeds is determined by using methods recommended by theWestern Canada Canola/Rapeseed Recommending Committee (WCC/RRC). 1 = low(less than 8 ppm), 2 = medium (8 to ppm), 3 = high (greater than 15ppm). Also, chlorophyll could be analyzed using NIR (Near Infrared)spectroscopy as long as the instrument is calibrated according to themanufacturer’s specifications.

The term “CMS” means the abbreviation for cytoplasmic male sterility.

The term “Cotyledon” means the cotyledon being a part of the embryowithin the seed of a plant; it is also referred to as a seed leaf. Upongermination, the cotyledon may become the embryonic first leaf of aseedling.

The term “Cotyledon Length” means the distance between the indentationat the top of the cotyledon and the point where the width of the petioleis approximately 4 mm.

The term “Cotyledon Width” means the width at the widest point of thecotyledon when the plant is at the two to three-leaf stage ofdevelopment. 3 = narrow, 5 = medium, 7 = wide. The term “CV%” means theabbreviation for coefficient of variation.

The term “Disease Resistance” means the resistance to various diseasesis evaluated and is expressed on a scale of 0 = not tested, 1 =resistant, 3 = moderately resistant, 5 = moderately susceptible, 7 =susceptible, and 9 = highly susceptible.

The term “Erucic Acid Content” means the percentage of the fatty acidsin the form of C22:1 as determined by one of the methods recommended bythe WCC/RRC, being AOCS Official Method Ce 2-66 Preparation of Methylesters of Long-Chain Fatty Acids or AOCS Official Method Ce 1-66 FattyAcid Composition by Gas Chromatography.

The term “Fatty Acid Content” means the typical percentages by weight offatty acids present in the endogenously formed oil of the mature wholedried seeds are determined. During such determination the seeds arecrushed and are extracted as fatty acid methyl esters following reactionwith methanol and sodium methoxide. Next the resulting ester is analyzedfor fatty acid content by gas liquid chromatography using a capillarycolumn which allows separation on the basis of the degree ofunsaturation and fatty acid chain length. This procedure is described inthe work of Daun, et al., (1983) J. Amer. Oil Chem. Soc. 60:1751 to1754.

The term “Flower Bud Location” describes the determination to be madewhether typical buds are disposed above or below the most recentlyopened flowers.

The term “Flower Date 50%” (Same as Time to Flowering) describes thenumber of days from planting until 50% of the plants in a planted areahave at least one open flower.

The term “Flower Petal Coloration” means the coloration of open exposedpetals on the first day of flowering is observed.

The term “Frost Tolerance (Spring Ty pe Only)” means the ability ofyoung plants to withstand late spring frosts at a typical growing areais evaluated and is expressed on a scale of 1 (poor) to 5 (excellent).

The term “Gene Silencing” means the interruption or suppression of theexpression of a gene at the level of transcription or translation. Theterm “Genotype” refers to the genetic constitution of a cell ororganism.

The term “Glucosinolate Content” means the total glucosinolates of seedat 8.5% moisture, as measured by AOCS Official Method AK-1-92(determination of glucosinolates content in rapeseed-colza by HPLC), isexpressed as micromoles per gram of defatted, oil-free meal. Capillarygas chromatography of the trimethylsityl derivatives of extracted andpurified desulfoglucosinolates with optimization to obtain optimumindole glucosinolate detection is described in “Procedures of theWestern Canada Canola/Rapeseed Recommending Committee Incorporated forthe Evaluation and R e com men d at ion for Registration ofCanola/Rapeseed Candidate Cultivars in Western Canada”. Also,glucosinolates could be analyzed using NIR (Near Infrared) spectroscopyas long as the instrument is calibrated according to the manufacturer’sspecifications.

The term “Grain” means the seed produced by the plant or a self or sibof the plant that is intended for food or feed use.

The term “Green Seed” means the number of seeds that are distinctlygreen throughout as defined by the Canadian Grain Commission expressedas a percentage of seeds tested.

The term “Herbicide Resistance” means the resistance to variousherbicides when applied at standard recommended application rates isexpressed on a scale of 1 (resistant), 2 (tolerant), or 3 (susceptible).

The term “Leaf Anthocyanin Coloration” means the presence or absence ofleaf anthocyanin coloration, and the degree thereof if present, areobserved when the plant has reached the 9- to 11-leaf stage.

The term “Leaf Attachment to Stem” means the presence or absence ofclasping where the leaf attaches to the stem, and when present thedegree thereof, are observed.

The term “Leaf Attitude” means the disposition of typical leaves withrespect to the petiole is observed when at least 6 leaves of the plantare formed.

The term “Leaf Color” means the leaf blade coloration is observed whenat least six leaves of the plant are completely developed.

The term “Leaf Glaucosity” means the presence or absence of a finewhitish powdery coating on the surface of the leaves, and the degreethereof when present, are observed. The term “Leaf Length” means thelength of the leaf blades and petioles are observed when at least sixleaves of the plant are completely developed.

The term “Leaf Lobes” means the fully developed upper stem leaves areobserved for the presence or absence of leaf lobes when at least 6leaves of the plant are completely developed.

The term “Leaf Margin Indentation” means the rating of the depth of theindentations along the upper third of the margin of the largest leaf. 1= absent or very weak (very shallow), 3 = weak (shallow), 5 = medium, 7= strong (deep), 9 = very strong (very deep).

The term “Leaf Margin Hairiness” means the leaf margins of the firstleaf are observed for the presence or absence of pubescence, and thedegree thereof, when the plant is at the two leaf-stage.

The term “Leaf Margin Shape” means the visual rating of the indentationsalong the upper third of the margin of the largest leaf. 1 = undulating,2 = rounded, 3 = sharp. The term “Leaf Surface” means the leaf surfaceis observed for the presence or absence of wrinkles when at least sixleaves of the plant are completely developed.

The term “Leaf Tip Reflexion” means the presence or absence of bendingof typical leaf tips and the degree thereof, if present, are observed atthe six to eleven leaf-stage.

The term “Leaf Upper Side Hairiness” means the upper surfaces of theleaves are observed for the presence or absence of hairiness, and thedegree thereof if present, when at least six leaves of the plant areformed.

The term “Leaf Width” means the width of the leaf blades is observedwhen at least six leaves of the plant are completely developed.

The term “Locus” means a specific location on a chromosome.

The term “Locus Conversion” means a locus conversion refers to plantswithin a variety that have been modified in a manner that retains theoverall genetics of the variety and further comprises one or more lociwith a specific desired trait, such as male sterility, insect, diseaseor herbicide resistance. Examples of single locus conversions includemutant genes, transgenes and native traits finely mapped to a singlelocus. One or more locus conversion traits may be introduced into asingle Canola variety.

The term “Lodging Resistance” means the resistance to lodging atmaturity is observed. 1 = not tested, 3 = poor, 5 = fair, 7 = good, 9 =excellent.

The term “LSD” is the abbreviation for least significant difference.

The term “Maturity” means the number of days from planting to maturityis observed, with maturity being defined as the plant stage when podswith seed change color, occurring from green to brown or black, on thebottom third of the pod-bearing area of the main stem.

The term “NMS” is the abbreviation for nuclear male sterility.

The term “Number of Leaf Lobes” means the frequency of leaf lobes, whenpresent, is observed when at least six leaves of the plant arecompletely developed.

The term “Oil Content” means the typical percentage by weight oilpresent in the mature whole dried seeds is determined by ISO 10565:1993Oilseeds Simultaneous determination of oil and water - Pulsed NMRmethod. Also, oil could be analyzed using NIR (Near Infrared)spectroscopy as long as the instrument is calibrated according to themanufacturer’s specifications, reference AOCS Procedure Am 1-92Determination of Oil, Moisture and Volatile Matter, and Protein byNear-Infrared Reflectance.

The term “Pedicel Length” means the typical length of the silique stemwhen mature is observed. 3 = short, 5 = medium, 7 = long.

The term “Petal Length” means the lengths of typical petals of fullyopened flowers are observed. 3 = short, 5 = medium, 7 = long. The term“Petal Width” means the widths of typical petals of fully opened flowersare observed. 3 = short, 5 = medium, 7 = long.

The term “Petiole Length” means the length of the petioles is observed,in a line forming lobed leaves, when at least six leaves of the plantare completely developed. 3 = short, 5 = medium, 7 = long.

The term “Plant Height” means the overall plant height at the end offlowering is observed. 3 = short, 5 = medium, 7 = tall.

The term “Ploidy” refers to the number of chromosomes exhibited by theline, for example diploid or tetraploid.

The term “Pod Anthocyanin Coloration” means the presence or absence atmaturity of silique anthocyanin coloration, and the degree thereof ifpresent, are observed.

The term “Pod (Silique) Beak Length” means the typical length of thesilique beak when mature is observed. 3 = short, 5 = medium, 7 = long.The term “Pod Habit” means the typical manner in which the siliques areborne on the plant at maturity is observed.

The term “Pod (Silique) Length” means the typical silique lengthobserved. 1 = short (less than 7 cm), 5 = medium (7 to 10 cm), 9 = long(greater than 10 cm).

The term “Pod (Silique) Attitude” means a visual rating of the anglejoining the pedicel to the pod at maturity. 1 = erect, 3 = semi-erect, 5= horizontal, 7 = semi-drooping, 9 = drooping.

The term “Pod Type” means the overall configuration of the siliqueobserved.

The term “Pod (Silique) Width” means the typical pod width observed whenmature. 3 = narrow (3 mm), 5 = medium (4 mm), 7 = wide (5 mm).

The term “Pollen Formation” means the relative level of pollen formationobserved at the time of dehiscence.

The term “Protein Content” means the typical percentage by weight ofprotein in the oil free meal of the mature whole dried seeds whendetermined by AOCS Official Method Ba4e-93 Combustion Method for theDetermination of Crude Protein. Also, protein could be analyzed usingNIR (Near Infrared) spectroscopy as long as the instrument is calibratedaccording to the manufacturer’s specifications, reference AOCS ProcedureAm 1-92 Determination of Oil, Moisture and Volatile Matter, and Proteinby Near-Infrared Reflectance.

The term “Resistance” means the ability of a plant to withstand exposureto an insect, disease, herbicide, or other condition. A resistant plantvariety or hybrid will have a level of resistance higher than acomparable wild-type variety or hybrid.

“Tolerance” is a term commonly used in crops affected by abiotic stress,diseases or pests and is used to describe an improved level of fieldresistance.

The term “Root Anthocyanin Coloration” means the presence or absence ofanthocyanin coloration in the skin at the top of the root, observed whenthe plant has reached at least the six- leaf stage.

The term “Root Anthocyanin Expression” means that when anthocyanincoloration is present in skin at the top of the root, it further isobserved for the exhibition of a reddish or bluish cast within suchcoloration when the plant has reached at least the six-leaf stage.

The term “Root Anthocyanin Streaking” means when anthocyanin colorationis present in the skin at the top of the root, it further is observedfor the presence or absence of streaking within such coloration when theplant has reached at least the six-leaf stage.

The term “Root Chlorophyll Coloration” means the presence or absence ofchlorophyll coloration in the skin at the top of the root is observedwhen the plant has reached at least the six-leaf stage.

The term “Root Coloration Below Ground” means the coloration of the rootskin below ground observed when the plant has reached at least thesix-leaf stage. Root Depth in Soil″ means the typical root depth isobserved when the plant has reached at least the six-leaf stage.

The term “Root Flesh Coloration” means the internal coloration of theroot flesh observed when the plant has reached at least the six-leafstage. The term “SE” means the abbreviation for standard error.

The term “Seedling Growth Habit” means the growth habit of youngseedlings is observed for the presence of a weak or strong rosettecharacter. 1 = weak rosette, 9 = strong rosette.

The term “Seeds Per Pod” means the average number of seeds per pod isobserved.

The term “Seed Coat Color” means the seed coat color of typical matureseeds is observed. 1 = black, 2 = brown, 3 = tan, 4 = yellow, 5 = mixed,6 = other.

The term “Seed Coat Mucilage” means the presence or absence of mucilageon the seed coat is determined and is expressed on a scale of 1 (absent)to 9 (present). During such determination a petri dish is filled to adepth of 0.3 cm. with water provided at room temperature. Seeds areadded to the petri dish and are immersed in water where they are allowedto stand for five minutes. The contents of the petri dish containing theimmersed seeds are then examined under a stereo microscope equipped withtransmitted light. The presence of mucilage and the level thereof isobserved as the intensity of a halo surrounding each seed.

The term “Seed Size” means the weight in grams of 1,000 typical seeds isdetermined at maturity while such seeds exhibit a moisture content ofapproximately 5 to 6 percent by weight.

The term “Shatter Resistance” means the resistance to silique shatteringis observed at seed maturity. 1 = not tested, 3 = poor, 5 = fair, 7 =good, 9 = does not shatter.

The term “SI” is the abbreviation for self-incompatible.

The term ″Speed of Root Formation″ means the typical speed of rootformation observed when the plant has reached the four to eleven-leafstage.

The term “SSFS” is the abbreviation for Sclerotinia sclerotiorum FieldSeverity score, a rating based on both percentage infection and diseaseseverity.

The term “Stem Anthocyanin Intensity” means the presence or absence ofleaf anthocyanin coloration and the intensity thereof, if present, areobserved when the plant has reached the nine to eleven-leaf stage. 1 =absent or very weak, 3 = weak, 5 = medium, 7 = strong, 9 = very strong.

The term “Stem Lodging” at Maturity means a visual rating of a plant’sability to resist stem lodging at maturity. 1 = very weak (lodged}, 9 =very strong (erect).

The term “Time to Flowering” means the determination of the number ofdays when at least 50 percent of the plants have one or more open budson a terminal raceme in the year of sowing.

The term “Seasonal Type” means whether the new line is considered to beprimarily a Spring or Winter type of Canola.

The term “Winter Survival (Winter Type Only)” means the ability towithstand winter temperatures at a typical growing area is evaluated andis expressed on a scale of 1 (poor) to 5 (excellent).

DETAILED DESCRIPTION

Breeding of new varieties, lines and hybrids is achieved by usingtechniques of mutagenesis, crossing and selection on a set of parentallines taking advantage of the plant’s method of pollination (self-, sib-or cross-pollination). Within such a breeding program the breederperforms multiple rounds of mutagenesis, crossing and selection withouthaving necessarily control of the results on a cellular or molecularlevel. After each round the breeder will select the germplasm for thenext round. Environmental factors like climate, soil and location willinfluence in addition to the unique genetic basis of each parent line ofevery round to the results of the breeding process. Consequently themolecular, physiological and anatomical characteristics of the resultingnew varieties, lines or hybrids cannot be predicted due to the hugeamount of possible genetic combinations. Consequently high efforts inbreeding are needed a develop new and superior Canola varieties Commontechniques in Canola breeding programs includes but is not limited totechniques such as mass selection, backcrossing, pedigree breeding andhaploidy (see Downey and Rakow, (1987) “Rapeseed and Mustard” In:Principles of Cultivar Development, Fehr, (ed.), pp 437-486; New York;Macmillan and Co.; Thompson, (1983) “Breeding winter oilseed rapeBrassica napus”; Advances in Applied Biology 7:1-104; and Ward, et. al.,(1985) Oilseed Rape, Farming Press Ltd., Wharfedale Road, Ipswich,Suffolk). By selecting recurrently populations of either self- orcrosspollinating Canola parent varieties based on their superiorcharacteristics the Canola plants are improved and are then further usedfor intercrossing to produce a new population to ensure thatquantitatively inherited traits controlled by numerous genes areimproved. For a simply inherited, highly heritable trait backcrossbreeding (i.e., recurrent crossing of the same parent after the firsttransfer crossing) can be used to transfer genes from the donor patentinto another line that serves as the recurrent parent. This approach hasbeen used for breeding disease resistant phenotypes of many plantspecies, and has been used to transfer low erucic acid and lowglucosinolate content into lines and breeding populations of Canola.Pedigree breeding and recurrent selection breeding methods are used todevelop varieties from breeding populations. Pedigree breeding startswith the crossing of two genotypes, each of which may have one or moredesirable characteristics that is lacking in the other or whichcomplements the other. If the two original parents do not provide all ofthe desired characteristics, other sources can be included in thebreeding population. In the pedigree method, superior plants are selfedand selected in successive generations. In the succeeding generationsthe heterozygous condition gives way to homogeneous lines as a result ofself-pollination and selection. Typically in the pedigree method ofbreeding, five or more generations of selfing and selection arepracticed: F1 to F2; F2 to F3; F3 to F4; F4 to F5, etc. For example, twoparents that are believed to possess favorable complementary traits arecrossed to produce an F1. An F2 population is produced by selfing one orseveral F1′s or by intercrossing two F1′s (i.e., sib mating). Selectionof the best individuals may begin in the F2 population, and beginning inthe F3 the best individuals in the best families are selected.Replicated testing of families can begin in the F4 generation to improvethe effectiveness of selection for traits with low heritability. At anadvanced stage of inbreeding (i.e., F5 and F1), the best lines ormixtures of phenotypically similar lines commonly are tested forpotential release as new cultivars. Backcrossing may be used inconjunction with pedigree breeding; for example, a combination ofbackcrossing and pedigree breeding with recurrent selection has beenused to incorporate blackleg resistance into certain cultivars ofBrassica napus. Plants that have been self-pollinated and selected fortype for many generations become homozygous at almost all gene loci andproduce a uniform population of true breeding progeny. If desired,double-haploid methods can also be used to extract homogeneous lines. Across between two different homozygous lines produces a uniformpopulation of hybrid plants that may be heterozygous for many gene loci.A cross of two plants each heterozygous at a number of gene loci willproduce a population of hybrid plants that differ genetically and willnot be uniform. The choice of breeding or selection methods depends onthe mode of plant reproduction, the heritability of the trait(s) beingimproved, and the type of cultivar used commercially, such as F1 hybridvariety or open pollinated variety. A true breeding homozygous line canalso be used as a parental line (inbred line) in a commercial hybrid. Ifthe line is being developed as an inbred for use in a hybrid, anappropriate pollination control system should be incorporated in theline. Suitability of an inbred line in a hybrid combination will dependupon the combining ability (general combining ability or specificcombining ability) of the inbred. Various breeding procedures are alsoutilized with these breeding and selection methods. The single-seeddescent procedure in the strict sense refers to planting a segregatingpopulation, harvesting a sample of one seed per plant, and using theone-seed sample to plant the next generation. When the population hasbeen advanced from the F2 to the desired level of inbreeding, the plantsfrom which lines are derived will each trace to different F2individuals. The number of plants in a population declines eachgeneration due to failure of some seeds to germinate or some plants toproduce at least one seed. As a result, not all of the F2 plantsoriginally sampled in the population will be represented by a progenywhen generation advance is completed. In a multiple-seed procedure,canola breeders commonly harvest one or more pods from each plant in apopulation and thresh them together to form a bulk. Part of the bulk isused to plant the next generation and part is put in reserve. Theprocedure has been referred to as modified single-seed descent or thepod-bulk technique. The multiple-seed procedure has been used to savelabor at harvest. It is considerably faster to thresh pods with amachine than to remove one seed from each by hand for the single-seedprocedure. The multiple-seed procedure also makes it possible to plantthe same number of seeds of a population each generation of inbreeding.Enough seeds are harvested to make up for those plants that did notgerminate or produce seed. If desired, doubled-haploid methods can beused to extract homogeneous lines. Molecular markers, includingtechniques such as Isozyme Electrophoresis, Restriction Fragment LengthPolymorphisms (RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs),Arbitrarily Primed Polymerase Chain Reaction (APPCR), DNA AmplificationFingerprinting (OAF), Sequence Characterized Amplified 10 Regions(SCARs), Amplified Fragment Length Polymorphisms (AFLPs), SimpleSequence Repeats (SSRs) and Single Nucleotide Polymorphisms (SNPs), maybe used in plant breeding methods. One use of molecular markers isQuantitative Trait Loci (QTL) mapping. QTL mapping is the use of markerswhich are known to be closely linked to alleles that have measurableeffects on a quantitative trait.

Selection in the breeding process is based upon the accumulation ofmarkers linked to the positive effecting alleles and/or the eliminationof the markers linked to the negative effecting alleles in the plant’sgenome. Molecular markers can also be used during the breeding processfor the selection of qualitative traits. For example, markers closelylinked to alleles or markers containing sequences within the actualalleles of interest can be used to select plants that contain thealleles of interest during a backcrossing breeding program. The markerscan also be used to select for the genome of the recurrent parent andagainst the markers of the donor parent. Using this procedure canminimize the amount of genome from the donor parent that remains in theselected plants. It can also be used to reduce the number of crossesback to the recurrent parent needed in a backcrossing program. The useof molecular markers in the selection process is often called GeneticMarker Enhanced Selection or Marker Assisted Selection (MAS). Theproduction of doubled haploids can also be used for the development ofinbreds in the breeding program. In Brassica napus, microspore culturetechnique is used in producing haploid embryos. The haploid embryos arethen regenerated on appropriate media as haploid plantlets, doublingchromosomes of which results in doubled haploid plants. This can beadvantageous because the process omits the generations of selfing neededto obtain a homozygous plant from a heterozygous source.

The development of a canola hybrid in a canola plant breeding programinvolves three steps: (1) the selection of plants from various germplasmpools for initial breeding crosses; (2) the selfing of the selectedplants from the breeding crosses for several generations to produce aseries of inbred lines, which, although different from each other, breedtrue and are highly uniform; and (3) crossing the selected inbred lineswith different inbred lines to produce the hybrids. During theinbreeding process in canola, the vigor of the lines decreases. Vigor isrestored when two different inbred lines are crossed to produce thehybrid. An important consequence of the homozygosity and homogeneity ofthe inbred lines is that the hybrid between a defined pair of inbredswill always be the same. Once the inbreds that give a superior hybridhave been identified, the hybrid seed can be reproduced indefinitely aslong as the homogeneity of the inbred parents is maintained.

Controlling Self-Pollination

Canola varieties are mainly self-pollinated; therefore, self-pollinationof the parental varieties must be controlled to make hybrid developmentfeasible. In developing improved new Brassica hybrid varieties, breedersmay use self- incompatible (SI), cytoplasmic male sterile (CMS) ornuclear male sterile (NMS) Brassica plants as the female parent. Inusing these plants, breeders are attempting to improve the efficiency ofseed production and the quality of the F1 hybrids and to reduce thebreeding costs. When hybridization is conducted without using SI, CMS orNMS plants, it is more difficult to obtain and isolate the desiredtraits in the progeny (F1 generation) because the parents are capable ofundergoing both crosspollination and self-pollination. If one of theparents is a SI, CMS or NMS plant that is incapable of producing pollen,only cross pollination will occur. By eliminating the pollen of oneparental variety in a cross, a plant breeder is assured of obtaininghybrid seed of uniform quality, provided that the parents are of uniformquality and the breeder conducts a single cross. In one instance,production of F 1 hybrids includes crossing a NMS Brassica female parentwith a pollen-producing male Brassica parent. To reproduce effectively,however, the male parent of the F1 hybrid must have a fertility restorergene (Rf gene). The presence of an Rf gene means that the F1 generationwill not be completely or partially sterile, so that eitherself-pollination or cross pollination may occur. Self-pollination of theF1 generation to produce several subsequent generations is important toensure that a desired trait is heritable and stable and that a newvariety has been isolated. One approach to ensure cross-pollination isthe male sterility system established in the Seedlink™ technology (WO-A89/10396).

Seedlink™ uses the transgenic expression of three different proteins inthe respective plant. The first protein Barnase is an extracellularribonuclease isolated from Bacillus amyloliquifaciens. The enzyme isinhibited by its corresponding intracellular inhibitor barstar (Hartley,Trends in Biochemical Sciences 1989, 14(11), 450). The DNA which codesfor Barnase has been introduced into Brassica using Agrobacteriumtransformation technology under the control of a tapetum specificpromoter and thereby leads to the suppression of the production offertile male gametes. Agrobacterium transformation is described in CA-A1 341 419. Together with Barnase gene being therefore a male sterilitygene also a gene coding for a phosphinothricin-N-acetyltransferase (PAT)enzyme isolated from Streptomyces hygroscopicus has been introduced intoBrassica using Agrobacterium transformation resulting in a male sterilefemale line resulting from the Ms8 event. Transgenic plants expressingthe PAT gene are described in WO-A 87/05629. The expression of the PATgene in a plant provides the plant with the ability to detoxify theherbicide Glufosinate. Glufosinate or its ammonium salt DLphosphinotricin is a broad spectrum herbicide and desiccant as itinhibits glutamine synthetase thereby leading to toxic ammoniumaccumulation in the plant. Plants which have been transformed with thePAT gene are able to acetylate the herbicide and thereby detoxify itinto an inactive compound. Therefore these plants are resistant toGlufosinate.

In order to restore fertility for producing the hybrid F1 population thefertility restorer line was produced by the introduction of the barstargene also under the control of a tapetum specific promoter together withthe PAT gene using Agrobacterium transformation as described in WO-A87/05629. The presence of the PAT gene in the transformants both for themale sterile and restorer lines can therefore serve as a marker for thesuccessful transformation and provides the herbicide resistance.Restorer lines typically comprise the RF3 event. In order to produce theF1 hybrid generation the female line being male sterile comprising theMs8 event is crossed with the male fertile restorer line comprising theRF3 event resulting in a F1 population carrying MS8/RF3.

Shatter Resistance

Reducing the shattering of the pods in Brassicaceae is desirable traitas high pod shatter resistance reduces yield losses during harvest orhigh winds due the unwanted shattering of pods. Pod shatter resistancemight be found in varying levels within Brassicaceae. Several relevantgenes important in controlling shatter resistance have been identified,e.g., SHATTERPROOF1 and SHATTERPROOF2 (Liljegren et al., 2000, Nature404, 766-770), the INDEHISCENT gene IND1 (Liljegren et al., 2004, Cell116: 843-853; PCT publication WO 01/79517) or the ALCATRAZ gene ALC1(Rajani et al. 2001, Current Biology 11, 1914-1922). Mutant alleles ofthese genes may be used to modify and improve shatter resistance inBrassicaceae, in particular in Canola hybrids as described in CA-A2,730,859.

Disease Resistance

Several diseases of Brassicaceae, in particular Brassica napus andhybrids thereof, are of high importance and are also addressed bybreeding approaches.

Blackleg, also known as stem cancer, is caused by Leptospheria maculans.The fungus infects the plants late in the season and may causesignificant yield losses. The fungus overwinters in infected plantdebris in the field and then infects lower stem and upper root partsleading to wide spread infections in susceptible Brassica plants.Blackleg is managed to a large extent through genetic resistance;however constant efforts in breeding in order to face the challenges bynew races of the fungus overcoming established resistance.

Clubroot, caused by the protist Plasmodiophora brassicae, has become asignificant thread to Brassicaceae, in particular in Western Canada. Thepathogen causes swellings on the root which ultimately leads topremature death of the plant. Due to long survival time-up to 20years—of resting spores in the soil, a field infected withPlasmodiophora brassicae will be impacted for a long time. In addition,movement of infected soil, e.g. through farm equipment, often will leadto further spreading of the infection. Until now, options to controlPlasmodiophora brassicae by agrochemicals are practically non-existant,and the disease is mainly managed through extended crop rotation as wellas sanitation practices or restricting access to the field. Thus forclubroot, genetic resistance could be key in managing the disease;however, knowledge on clubroot races and associated resistance genes islimited. Breeding for clubroot resistance is therefore an essential taskand objective in Brassicaceae breeding.

Hybrid Development

9CN0089 is a fully restored spring Brassica napus hybrid with aglufosinate resistance gene, based on SeedLink hybridization system asdescribed above. It was developed at the Breeding Centre of BASF CanadaInc. in Saskatoon, Canada. It is a single cross hybrid produced bycrossing a female parent expressing the PAT and the barnase gene underthe control of a tapetum specific promoter by a restorer - male R lineexpressing the PAT and the barstar gene under the control of a tapetumspecific promoter. A pollination control system and effective transferof pollen from one parent to the other offers improved plant breedingand an effective method for producing hybrid canola seed and plants. Forexample, the Seedlink (NMS) system, developed using Agrobacteriumtransformation, is one of the most frequently used methods of hybridproduction. It provides stable expression of the male sterility traitand an effective restorer gene.

For most traits the true genotypic value may be masked by otherconfounding plant traits or environmental factors. One method foridentifying a superior plant is to observe its performance relative toother experimental plants and to one or more widely grown standardvarieties. If a single observation is inconclusive, replicatedobservations provide a better estimate of the genetic worth. Propertesting should detect any major faults and establish the level ofsuperiority or improvement over current varieties. In addition toshowing superior performance, there must be a demand for a new varietythat is compatible with industry standards or which creates a newmarket. The introduction of a new variety commonly will incur additionalcosts to the seed producer, the grower, the processor and the consumer,for special advertising and marketing, altered seed and commercialproduction practices, and new product utilization. The testing precedingrelease of a new variety should take into consideration research anddevelopment costs as well as technical superiority of the final variety.For seed-propagated varieties, it must be feasible to produce seedeasily and economically. These processes, which lead to the final stepof marketing and distribution, usually take from approximately six totwelve years from the time the first cross is made. Therefore, thedevelopment of new varieties is a time-consuming process that requiresprecise forward planning, efficient use of resources, and a minimum ofchanges in direction. Further, as a result of the advances in sterilitysystems, lines are developed that can be used as an open pollinatedvariety (i.e., a pureline cultivar sold to the grower for planting)and/or as a sterile inbred (female) used in the production of F1 hybridseed. In the latter case, favorable combining ability with a restorer(male) would be desirable. The resulting hybrid seed would then be soldto the grower for planting. Combining ability of a line, as well as theperformance of the line per se, is a factor in the selection of improvedcanola lines that may be used as inbreds. Combining ability refers to aline’s contribution as a parent when crossed with other lines to formhybrids. The hybrids formed for the purpose of selecting superior linesare designated test crosses. One way of measuring combining ability isby using breeding values. Breeding values are based on the overall meanof a number of test crosses. This mean is then adjusted to removeenvironmental effects and it is adjusted for known genetic relationshipsamong the lines. Hybrid seed production requires inactivation of pollenproduced by the female parent. Incomplete inactivation of the pollenprovides the potential for self-pollination. This inadvertentlyself-pollinated seed may be unintentionally harvested and packaged withhybrid seed. Similarly, because the male parent is grown next to thefemale parent in the field, there is also the potential that the maleselfed seed could be unintentionally harvested and packaged with thehybrid seed. Once the seed from the hybrid bag is planted, it ispossible to identify and select these self-pollinated plants. Theseself-pollinated plants will be genetically equivalent to one of theinbred lines used to produce the hybrid. Though the possibility ofinbreds being included in hybrid seed bags exists, the occurrence israre because much care is taken to avoid such inclusions. Theseself-pollinated plants can be identified and selected by one skilled inthe art, through either visual or molecular methods. Brassica napuscanola plants, absent the use of sterility systems, are recognized tocommonly be self-fertile with approximately 70 to 90 percent of the seednormally forming as the result of self-pollination. The percentage ofcross pollination may be further enhanced when populations of recognizedinsect pollinators at a given growing site are greater. Thus openpollination is often used in commercial canola production. Since canolavariety 9CN0089 is a hybrid produced from substantially homogeneousparents, it can be reproduced by planting seeds of such parents, growingthe resulting canola plants under controlled pollination conditions withadequate isolation so that cross-pollination occurs between the parents,and harvesting the resulting hybrid seed using conventional agronomicpractices. Locus Conversions of Canola Variety 9CN0089 represents a newbase genetic line into which a new locus or trait may be introduced.Direct transformation and backcrossing represent two important methodsthat can be used to accomplish such an introgression. The term locusconversion is used to designate the product of such an introgression. Toselect and develop a superior hybrid, it is necessary to identify andselect genetically unique individuals that occur in a segregatingpopulation. The segregating population is the result of a combination ofcrossover events plus the independent assortment of specificcombinations of alleles at many gene loci that results in specific andunique genotypes. Once such a variety is developed its value to societyis substantial since it is important to advance the germplasm base as awhole in order to maintain or improve traits such as yield, diseaseresistance, pest resistance and plant performance in extreme weatherconditions. Locus conversions are routinely used to add or modify one ora few traits of such a line and this further enhances its value andusefulness to society. Backcrossing can be used to improve inbredvarieties and a hybrid variety which is made using those inbreds.Backcrossing can be used to transfer a specific desirable trait from onevariety, the donor parent, to an inbred called the recurrent parentwhich has overall good agronomic characteristics yet that lacks thedesirable trait. This transfer of the desirable trait into an inbredwith overall good agronomic characteristics can be accomplished by firstcrossing a recurrent parent to a donor parent (non-recurrent parent).The progeny of this cross is then mated back to the recurrent parentfollowed by selection in the resultant progeny for the desired trait tobe transferred from the non-recurrent parent. Traits may be used bythose of ordinary skill in the art to characterize progeny. In oneaspect a locus converted plant cell of a locus converted plant isdescribed which is obtained by introducing a locus conversion intoCanola hybrid variety 9CN0089, and wherein the locus converted plantcell is identical to a cell from variety 9CN0089 except for the locusconversion and the locus converted plant expresses essentially thephysiological and morphological characteristics of Canola hybrid variety9CN0089. In another aspect the locus conversion confers a trait and thetrait is selected from the group comprising male sterility,site-specific recombination, abiotic stress resistance, alteredphosphate, altered antioxidants, altered fatty acids, altered essentialamino acids, altered carbohydrates, improved shatter resistance,improved lodging, herbicide resistance, insect resistance or diseaseresistance.

Traits are commonly evaluated at a significance level, such as a 1 %, 5%or 10% significance level, when measured in plants grown in the sameenvironmental conditions. For example, a locus conversion of 9CN0089 maybe characterized as having essentially the same phenotypic traits as9CN0089. Molecular markers can also be used during the breeding processfor the selection of qualitative traits. For example, markers can beused to select plants that contain the alleles of interest during abackcrossing breeding program. The markers can also be used to selectfor the genome of the recurrent parent and against the genome of thedonor parent. Using this procedure can minimize the amount of genomefrom the donor parent that remains in the selected plants. A locusconversion of 9CN0089 will retain the genetic integrity of 9CN0089. Alocus conversion of 9CN0089 will comprise at least 92%, 93%, 94%, 95%,96%, 97%, 98% or 20 99% of the base genetics of 9CN0089. For example, alocus conversion of 9CN0089 can be developed when DNA sequences areintroduced through backcrossing (Hallauer et al., 1988), with a parentof 9CN0089 utilized as the recurrent parent. Both naturally occurringand transgenic DNA sequences may be introduced through backcrossingtechniques. A backcross conversion may produce a plant with a locusconversion in at least one or more backcrosses, including at least 2crosses, at least 3 crosses, at least 4 crosses, at least 5 crosses andthe like. Molecular marker assisted breeding or selection may beutilized to reduce the number of backcrosses necessary to achieve thebackcross conversion. For example, see Openshaw, S.J. et al., Markerassisted Selection in Backcross Breeding. In: Proceedings Symposium ofthe 30 Analysis of Molecular Data, August 1994, Crop Science Society ofAmerica, Corvallis, OR, where it is demonstrated that a backcrossconversion can be made in as few as two backcrosses.

In another aspect a plant cell of an Essentially Derived Variety of9CN0089 having one, two or three physiological and/or morphologicalcharacteristics which are different from those of 9CN0089 and whichotherwise has all the physiological and morphological characteristics of9CN0089 is described.

In another aspect the invention provides for a Canola hybrid variety9CN0089. The invention also provides for a plurality of seeds of the newvariety, plants produced from growing the seeds of the new variety9CN0089, and progeny of any of these. Especially, progeny retaining oneor more (or all) of the “distinguishing characteristics” or one or more(or all) of the “essential morphological and physiologicalcharacteristics” or essentially all physiological and morphologicalcharacteristics of 9CN0089 referred to herein, are encompassed herein aswell as methods for producing these.

In one aspect, such progeny have (essentially) all the physiological andmorphological characteristics of Canola hybrid variety 9CN0089 whengrown under the same environmental conditions.

Further, Canola seeds produced on a plant grown from these seeds isprovided.

In yet another embodiment of the invention, an Essentially DerivedVariety of Canola hybrid variety 9CN0089 having one, two or threephysiological and/or morphological characteristics which are differentfrom those of 9CN0089 and which otherwise has all the physiological andmorphological characteristics of 9CN0089, wherein a representativesample of seed of variety 9CN0089 has been deposited under AccessionNumber [XXXXXX] is provided.

A plant having “(essentially) all the physiological and morphologicalcharacteristics” means a plant having the physiological andmorphological characteristics when grown under the same environmentalconditions of the plant from which it was derived, e.g. the progenitorplant, the parent, the recurrent parent, the plant used for tissue- orcell culture, etc. In certain embodiments the plant has all thephysiological and morphological characteristics, except for certaincharacteristics mentioned, e.g. the characteristic(s) derived from aconverted or introduced gene or trait and/or except for thecharacteristics which differ in an EDV. A plant have one or more“essential physiological and/or morphological characteristics” or one ormore “distinguishing characteristics” refers to a plant having (orretaining) one or more of the characteristics mentioned in Table 1 whengrown under the same environmental conditions that distinguish 9CN0089from the most similar varieties, such as but not limited to oil content,protein content, erucic acid content, glucosinolate content, time tomaturity, disease resistance/tolerance, in particular to the importantdisease blackleg and shatter resistance.

In other aspects, the invention provides for progeny of variety 9CN0089such as progeny obtained by further breeding 9CN0089. Further breeding9CN0089 includes selfing 9CN0089 one or more times and/orcross-pollinating 9CN0089 with another Canola plant or variety one ormore times. In particular, the invention provides for progeny thatretain all the essential morphological and physiological characteristicsof 9CN0089 or that retain one or more of the distinguishingcharacteristics of the Canola type described further above and whengrown under the same environmental conditions. In another aspect, theinvention provides for vegetative reproductions of the variety andessentially derived varieties (EDVs) of 9CN0089.

Uses of Canola

Currently Brassica napus canola is being recognized as an increasinglyimportant oilseed crop and a source of meal in many parts of the world.Therefore in one aspect the use of seeds of Canola hybrid variety9CN0089 is described to grow a commercial crop. The oil as removed fromthe seeds commonly contains a lesser concentration of endogenouslyformed saturated fatty acids than other vegetable oils and is wellsuited for use in the production of salad oil or other food products orin cooking or frying applications. The oil also finds utility inindustrial applications. Additionally, the meal component of the seedscan be used as a nutritious protein concentrate for livestock.

Canola oil has the lowest level of saturated fatty acids of allvegetable oils. “Canola” refers to rapeseed (Brassica) which (1) has anerucic acid (C22:1) content of at most 2 percent by weight based on thetotal fatty acid content of a seed, preferably at most 0.5 percent byweight and most preferably essentially 0 percent 5 by weight; and (2)produces, after crushing, an air-dried meal containing less than 30micromoles (µmol) glucosinolates per gram of defatted (oil-free) meal.These types of rapeseed are distinguished by their edibility incomparison to more traditional varieties of the species. In one aspectthe use of a Canola hybrid plant designated 9CN0089 is described toproduce a commodity product comprising seed oil, meal, fiber, orprotein. Also the described is the use of a Canola hybrid plantdesignated 9CN0089 to produce crushed non-viable F2 seed and the use ofsuch seeds to produce oil, meal, fiber, or protein.

Diseases, Pests and Weeds

Brassica, in particular Canola is infected by a number of microbialdiseases. The most important ones are listed below:

-   bacterial    -   bacterial leaf spot - Pseudomonas syringae    -   bacterial soft rot - Erwinia marginalis    -   bacterial soft rot Pseudomonas - Pseudomonas marginalis    -   black rot - Xanthomonas campestris-   fungal    -   Alternaria black spot - Alternaria spp.    -   anthracnose - Colletrotrichum higginsianum    -   blackleg - Leptosphaeria maculans    -   black mold rot - Rhizopus stolonifer    -   black root - Aphanomyces raphani    -   cercospora leaf spot - Cercospora brassicicola    -   clubroot - Plasmodiophora brassicae    -   downey mildew - Peronospora parasitica    -   fusarium wilt - Fusarium avenaceum and F. oxysporum.    -   graymold - Botrytis cinerea    -   light leaf spot - Pyrenopeziza brassicae    -   phymatotrichum root rot - Phymatotrichopsisomnivora    -   phytophthora root rot - Phytophthora megasperma    -   powdery mildew - Erysiphe polygoni    -   ring spot - Mycosphaerella brassicicola    -   root rot complex - Rhizoctonia solani, Fusarium and Pythium spp.    -   seedling disease complex - Rhizoctonia solani, Fusarium and        Pythium spp.    -   sclerotinia white stem rot - Sclerotinia sclerotiorum    -   southern blight - Sclerotium rolfsii    -   verticillium wilt - Verticillium albo-atrum    -   white leaf spot and gray stem - Pseudocercosporella capsellae    -   white rust and staghead - Albugo candida    -   yellows - Fusarium oxysporum-   viral    -   cauliflower mosaic virus    -   radish mosaic virus    -   turnip mosaic virus    -   beet Western yellows virus-   phytoplasma-like    -   aster yellows

These diseases cause significant yield losses both in quantity andquality of the crop each year. Creation of disease tolerant or resistantcanola cultivars has been an important goal for many of the Canadiancanola breeding organizations. Conventional methods for control ofdiseases include chemical control, disease resistance and culturalcontrol, each of which is described below.

Therefore in one aspect a method for the protection of a group ofcultivated plants of Canola hybrid variety 9CN0089 in a field isdescribed wherein the harmful microorganisms are controlled by theapplication of a composition comprising one or more microbicidal activeingredients. In one particular embodiment these active ingredients areselected from the group comprising azoxystrobin, benzovindiflupyr,boscalid, cyprodinil, fludioxonil, fluxapyroxad, fluopyram,ipfentrifluconazole, iprodione, isoflucypram, metalaxyl, mefenoxam,mefentrifluconazole, metconazole, penthiopyrad, picoxystrobin,propiconazole, prothioconazole, pyraclostrobin, pyraziflumid,pydiflumetofen, sedaxane, and tebuconazole. The active ingredients canbe applied to Canola hybrid variety 9CN0089 as a foliar or seedtreatment in customary formulations. The active ingredients can also beapplied to the soil, where the Canola hybrid variety 9CN0089 will beseeded, is seeded, is growing, will be harvested or is harvested.

A significant number of weeds are present when growing Brassicaceae, inparticular Canola.

The most important ones are listed below:

-   ball mustard-   barnyard grass-   bluebur-   Canada thistle-   chickweed-   cleavers-   common peppergrass-   cow cockle-   field horsetail-   flixweed-   green foxtail-   green smartweed-   hare’s ear mustard-   hemp nettle-   lady’s thumb-   lamb’s-quarters-   night-flowering catchfly-   quackgrass-   redroot pigweed-   Russian thistle-   shepherd’s purse-   sow thistle-   stinkweed-   stork’s bill-   volunteer canola-   wild buckwheat-   wild mustard-   wild oats-   wild rose-   wormseed mustard

Conventional methods for control of weeds include mainly chemical ormechanical control.

The following herbicides are suitable for controlling weeds inBrassicaceae, in particular Canola: Carfentrazone (e.g., marketed asAim™ by FMC), Clethodim (e.g., marketed as Centurion™ by BASF, as Arrow™by ADAMA, as Shadow™ by Loveland, as Select™ by Ayrsta), Amitrol (e.g.,marketed as Amitrol by Nufarm), Imazamox, Imazapyr (e.g., marketed asAres™ or Odyssey™ or Solo™ by BASF), Quizalofop-p-ethyl (e.g., marketedas Assure II™ by Dupont, Yuma GL™ by Gowan), Triallate (e.g., marketedas Avadex™ by Gowan or Fortress™ by Gowan), Clopyralid (e.g., marketedas Eclipse III A™ by Corteva, Lontrel™ by Corteva), Ethalfluralin (e.g.,marketed as Edge™ by Gowan), Trifluralin (e.g., marketed as Fortress™ byGowan), Glyphosate (e.g., marketed as Roundup Weathermax,™ Roundup Ultra2,™ Roundup Transorb™ by Monsanto), Saflufenacil (e.g., marketed asHeat™ by BASF), Glufosinate (e.g., marketed as Liberty™ by BASF),Quinclorac (e.g., marketed as Facet™ by BASF), Ethametsulfuron-methyl(e.g., marketed as Muster Toss-N-Go™ by Dupont), Tepraloxydim ((e.g.,marketed as Equinox™ by BASF), Sethoxydim (e.g., marketed as OdysseyUltra B™ or Poast Ultra™ by BASF), Diquat (e.g., marketed as Reglone™ bySyngenta), Trifluralin (e.g., marketed as Bonanza™ by Loveland, asRival™ by Nufarm, as Treflan™ by Gowan).

Therefore in one aspect a method for the protection of a group ofcultivated plants of Canola hybrid variety 9CN0089 in a field isdescribed wherein the weeds are controlled by the application of acomposition comprising at least one herbicidal active ingredients. Inone particular embodiment these active ingredients are selected from thegroup comprising amitrol, carfentrazone, clethodim, clopyralid, dicamba,diquat, ethalfluralin, ethametsulfuron-methyl, florasulam, imazamox,imazapyr, glufosinate, glufosinate-ammonium, glyphosate, MCPA amine,MCPA ester, metsulfuron, quizalofop-p-ethyl, quinclorac, saflufenacil,triallate, and trifluralin. The active ingredients can be applied as afoliar, a pre-emergent, a post-emergent, a pre-harvest, a post-harvest,or a pre-seeding application in customary formulations.

A significant number of insect pests are present when growingBrassicaceae, in particular Canola. These pests cause significant yieldlosses both in quantity and quality of the crop each year. Conventionalmethods for control of diseases include chemical control, pestresistance and cultural control.

The most important pests are listed below:

-   Autographia californica Speyer-   Aphids eg. Brevicoryne brassicae, Hyadaphis erysimi-   Loxostege sticticalis-   Mamestra configurata-   Ceutorhynus species, e.g., Ceutorhynchus obstrictis, Ceutorhynchus    assimilis-   Contarinia nasturtii Kieffer-   Dicestra trifolii-   Plutella xylostella-   Phyllotrella species, e.g., P. cruficerae, P. striolata-   Lygus species, e.g., Lygus lineolaris-   Vanessa cardui-   Entomoscelis americana Brown

Delia Species

The following insecticides are suitable for controlling pests inBrassicaeae in particular Canola:

Chlorantraniliprole (e.g., marketed as Lumivia™ by E. I. Du Pont),cyantraniliprole (e.g., marketed as Lumiderm™by E. I. Du Pont),Sulfoxaflor (e.g., marketed as Transform™ WG Corteva or as Rascendo™ bySyngenta), and spirotetramat.

Therefore in one aspect a method for the protection of a group ofcultivated plants of Canola hybrid variety 9CN0089 in a field whereinthe pests are controlled by the application of a composition comprisingone or more insecticidal active ingredients. In one particularembodiment these active ingredients are selected from the groupcomprising broflanilide, carbaryl, carbofuran, chlorantraniliprole,chlorpyrifos, cypermethrin, cyclaniliprole, cyhalodiamide, clothianidin,deltamethrin, dimethoate, cyantraniliprole, cyhalothrin-lambda,imidacloprid, lambda-cyhalothrin, permethrin, sulfoxaflor,spirotetramate, tetraniliprole, and thiamethoxam. The active ingredientscan be applied as a foliar or seed treatment in customary formulations.

Therefore in one aspect a method for the protection of a group ofcultivated plants of Canola hybrid variety 9CN0089 in a field isprovided wherein harmful microorganisms and/or pests are controlled bythe application of a composition comprising one or more fungicidal orinsecticidal active ingredients onto the seeds of said variety beforeseeding.

In one particular embodiment these active ingredients are selected fromthe group of comprising broflanilide, carbaryl, carbofuran,chlorantraniliprole, chlorpyrifos, cypermethrin, cyclaniliprole,cyhalodiamide, clothianidin, deltamethrin, dimethoate, cyantraniliprole,cyhalothrin-lambda, imidacloprid, lambda-cyhalothrin, permethrin,sulfoxaflor, spirotetramate, tetraniliprole, thiamethoxam azoxystrobin,benzovindiflupyr, boscalid, cyprodinil, fludioxonil, fluxapyroxad,fluopyram, ipfentrifluconazole, iprodione, isoflucypram, metalaxyl,mefenoxam, mefentrifluconazole, metconazole, penthiopyrad,picoxystrobin, propiconazole, prothioconazole, pyraclostrobin,pyraziflumid, pydiflumetofen, sedaxane, and tebuconazole.

Characteristics of 9CN0089

A canola hybrid needs to be homogenous and reproducible to be useful forthe production of a commercial crop on a reliable basis. There are anumber of analytical methods available to determine the phenotypicstability of a canola hybrid. The oldest and most traditional method ofanalysis is the observation of phenotypic traits. The data are usuallycollected in field experiments over the life of the canola plants to beexamined. Phenotypic characteristics most often are observed for traitsassociated with seed yield, seed oil content, seed protein content,fatty acid composition of oil, glucosinolate content of meal, growthhabit, lodging resistance, plant height, shatter resistance, etc. Inaddition to phenotypic observations, the genotype of a plant can also beexamined. A plant’s genotype can be used to identify plants of the samevariety or a related variety. For example, the genotype can be used todetermine the pedigree of a plant. There are many laboratory-basedtechniques available for the analysis, comparison and characterizationof plant genotype; among these are Isozyme Electrophoresis, RestrictionFragment Length Polymorphisms (RFLPs), Randomly Amplified PolymorphicDNAs (RAPDs), Arbitrarily Primed Polymerase Chain Reaction (AP-PCR), DNAAmplification Fingerprinting (OAF), Sequence Characterized AmplifiedRegions (SCARs), Amplified Fragment Length Polymorphisms (AFLPs), SimpleSequence Repeats (SSRs) which are also referred to as Microsatellites,and Single Nucleotide Polymorphisms (SNPs). The variety of the presentinvention has shown uniformity and stability for all traits, asdescribed in the following variety description information. The varietyhas been increased with continued observation for uniformity. 9CN0089 isan early maturing, high yielding, glufosinate resistant Brassica napuscanola hybrid having a resistant (R) rating for blackleg. Its oilcontent is equivalent to WCC/RRC checks. It can be distinguished fromthe checks by the petiole length, leaf width, and silique length. Table1 provides data on morphological, agronomic, and quality traits for9CN0089. When preparing the detailed phenotypic information thatfollows, plants of the new 9CN0089 variety were observed while beinggrown using conventional agronomic practices. For comparative purposes,canola plants of canola varieties PA8CN153 and PR8CN745 were similarlygrown in a replicated experiment. Observations were recorded on variousmorphological traits for the hybrid 9CN0089 and comparative checkcultivars. (See Table 1.) Hybrid 9CN0089 can be advantageously used inaccordance with the breeding methods described herein and those known inthe art to produce hybrids and other progeny plants retaining desiredtrait combinations of 9CN0089. This invention is thus also directed tomethods for producing a canola plant by crossing a first parent canolaplant with a second parent canola plant wherein either the first orsecond parent canola plant is canola variety 9CN0089. Further, bothfirst and second parent canola plants can come from the canola variety9CN0089. Either the first or the second parent plant may be malesterile. Still further, this invention also is directed to methods forproducing a 9CN0089-derived canola plant by crossing canola variety9CN0089 with a second canola plant and growing the progeny seed, andrepeating the crossing and growing steps with the canola 9CN0089-derivedplant from 1 to 2 times, 1 to 3 times, 1 to 4 times, or 1 to 5 times.Thus, any such methods using the canola variety 9CN0089 are part of thisinvention: open pollination, selfing, backcrosses, hybrid production,crosses to populations, and the like. All plants produced using canolavariety 9CN0089 as a parent are within the scope of this invention,including plants derived from canola variety 9CN0089. This includescanola lines derived from 9CN0089 which include components for eithermale sterility or for restoration of fertility. Advantageously, thecanola variety is used in crosses with other, different, canola plantsto produce first generation (F1) canola hybrid seeds and plants withsuperior characteristics. The invention also includes a single-geneconversion of 9CN0089. A single-gene conversion occurs when DNAsequences are introduced through traditional (non-transformation)breeding techniques, such as backcrossing. DNA sequences, whethernaturally occurring or transgenes, may be introduced using thesetraditional breeding techniques. Desired traits transferred through thisprocess include, but are not limited to, fertility restoration, fattyacid profile modification, other nutritional enhancements, industrialenhancements, disease resistance, insect resistance, herbicideresistance and yield enhancements. The trait of interest is transferredfrom the donor parent to the recurrent parent, in this case, the canolaplant disclosed herein. Single-gene traits may result from the transferof either a dominant allele or a recessive allele. Selection of progenycontaining the trait of interest is done by direct selection for a traitassociated with a dominant allele. Selection of progeny for a trait thatis transferred via a recessive allele will require growing and selfingthe first backcross to determine which plants carry the recessivealleles. Recessive traits may require additional progeny testing insuccessive backcross generations to determine the presence of the geneof interest. It should be understood that the canola variety of theinvention can, through routine manipulation by cytoplasmic genes,nuclear genes, or other factors, be produced in a male-sterile orrestorer form as described in the references discussed earlier. Suchembodiments are also within the scope of the present claims. Canolavariety 9CN0089 can be manipulated to be male sterile by any of a numberof methods known in the art, including by the use of mechanical methods,chemical methods, self-incompatibility (SI), cytoplasmic male sterility(CMS) (either Ogura or another system}, or nuclear male sterility (NMS).The term “manipulated to be male sterile” refers to the use of anyavailable techniques to produce a male sterile version of canola variety9CN0089. The male sterility may be either partial or complete malesterility. This invention is also directed to F1 hybrid seed and plantsproduced by the use of Canola variety 9CN0089. Canola variety 9CN0089can also further comprise a component for fertility restoration of amale sterile plant, such as an Rf restorer gene. In this case, canolavariety 9CN0089 could then be used as the male plant in hybrid seedproduction. This invention is also directed to the use of 9CN0089 intissue culture. As used herein, the term plant includes plantprotoplasts, plant cell tissue cultures from which canola plants can beregenerated, plant calli, plant clumps, and plant cells that are intactin plants or parts of plants, such as embryos, pollen, ovules, seeds,flowers, kernels, ears, cobs, leaves, husks, stalks, roots, root tips,anthers, silk and the like. Pauls, et al., (2006) (Canadian J of Botany84(4):668-678) confirmed that tissue culture as well as microsporeculture for regeneration of canola plants can be accomplishedsuccessfully. Chuong, et al., (1985) “A Simple Culture Method forBrassica Hypocotyl Protoplasts”, Plant Cell Reports 4:4-6; Barsby, etal., (Spring 1996) “A Rapid and Efficient Alternative Procedure for theRegeneration of Plants from Hypocotyl Protoplasts of Brassica napus”,Plant Cell Reports; Kartha, et al., (1974) “In vitro Plant Formationfrom Stem Explants of Rape”, Physiol. Plant 31:217-220; Narasimhulu, etal., (Spring 1988) “Species Specific Shoot Regeneration Response ofCotyledonary Explants of Brassicas”, Plant Cell Reports; Swanson, (1990)“Microspore Culture in Brassica”, Methods in Molecular Biology 6(17):159; “Cell Culture techniques and Canola improvement” J. Am. Oil Chem.Soc. 66(4):455-56 (1989). Thus, it is clear from the literature that thestate of the art is such that these methods of obtaining plants are, andwere, “conventional” in the sense that they are routinely used and havea very high rate of success.

The utility of canola variety 9CN0089 also extends to crosses with otherspecies. Commonly, suitable species will be of the family Brassicaceae.The advent of new molecular biological techniques has allowed theisolation and characterization of genetic elements with specificfunctions, such as encoding specific protein products. Scientists in thefield of plant biology developed a strong interest in engineering thegenome of plants to contain and express foreign genetic elements, oradditional, or modified versions of native or endogenous geneticelements in order to alter the traits of a plant in a specific manner.Any DNA sequences, whether from a different species, or from the samespecies that are inserted into the genome using transformation arereferred to herein collectively as “transgenes”. Over the last fifteento twenty years several methods for producing transgenic plants havebeen developed, and the present invention, in particular embodiments,also relates to transformed versions of the claimed canola variety9CN0089.

Numerous methods for plant transformation have been developed, includingbiological and physical plant transformation protocols. See, forexample, Miki, et al., “Procedures for Introducing Foreign DNA intoPlants” in Methods in Plant Molecular Biology and Biotechnology, Glick,and Genetic Transformation for the improvement of Canola World Conf,Biotechnol. Fats and Oils Ind. 43-46 (1988). In addition, expressionvectors and in vitro culture methods for plant cell or tissuetransformation and regeneration of plants are available. See, forexample, Gruber, et al., “Vectors for Plant Transformation” in Methodsin Plant Molecular Biology and Biotechnology, Glick and Thompson, Eds.(CRC Press, Inc., Boca Raton, 1993) pages 89-119. The most prevalenttypes of plant transformation involve the construction of an expressionvector. Such a vector comprises a DNA sequence that contains a geneunder the control of or operatively linked to a regulatory element, forexample a promoter. The vector may contain one or more genes and one ormore regulatory elements. A genetic trait which has been engineered intoa particular canola plant using transformation techniques could be movedinto another line using traditional breeding techniques that are wellknown in the plant breeding arts. For example, a backcrossing approachcould be used to move a transgene from a transformed canola plant to anelite inbred line and the resulting progeny would comprise a transgene.Also, if an inbred line was used for the transformation then thetransgenic plants could be crossed to a different line in order toproduce a transgenic hybrid canola plant. As used herein, “crossing” canrefer to a simple X by Y cross, or the process of backcrossing,depending on the context. Various genetic elements can be introducedinto the plant genome using transformation. These elements include butare not limited to genes; coding sequences; inducible, constitutive, andtissue specific promoters; enhancing sequences; and signal and targetingsequences. See U.S. Pat. Number 6,222,101. With transgenic plantsaccording to the present invention, a foreign protein can be produced incommercial quantities. Thus, techniques for the selection andpropagation of transformed plants, which are well understood in the art,yield a plurality of transgenic plants which are harvested in aconventional manner, and a foreign protein then can be extracted from atissue of interest or from total biomass. Protein extraction from plantbiomass can be accomplished by known methods which are discussed, forexample, by Heney and Orr, (1981) Anal. Biochem. 114:92-96. A geneticmap can be generated, primarily via conventional Restriction FragmentLength Polymorphisms (RFLP), Polymerase Chain Reaction (PCR) analysis,Simple Sequence Repeats (SSR), and Single Nucleotide Polymorphisms(SNPs), which identifies the approximate chromosomal location of theintegrated DNA molecule coding for the foreign protein. For exemplarymethodologies in this regard, see, Glick and Thompson, METHODS IN PLANTMOLECULAR BIOLOGY AND BIOTECHNOLOGY 269-284 (CRC Press, Boca Raton,1993). Map Io information concerning chromosomal location is useful forproprietary protection of a subject transgenic plant. If unauthorizedpropagation is undertaken and crosses made with other germplasm, the mapof the integration region can be compared to similar maps for suspectplants, to determine if the latter have a common parentage with thesubject plant. Map comparisons would involve hybridizations, RFLP, PCR,SSR, SNP, and sequencing, all of which are conventional techniques.Likewise, by means of the present invention, plants can be geneticallyengineered to express various phenotypes of agronomic interest.Exemplary transgenes implicated in this regard include, but are notlimited to, those categorized below.

1. Genes that confer resistance to pests or disease and that encode:

(A) Plant disease resistance genes. Plant defenses are often activatedby specific interaction between the product of a disease resistance gene(R) in the plant and the product of a corresponding avirulence (Avr)gene in the pathogen. A plant variety can be transformed with clonedresistance gene to engineer plants that are resistant to specificpathogen strains. See, for example Jones, et al., (1994) Science 266:789(cloning of the tomato Cf-9 gene for resistance to Cladosporium fulvum);Martin, et al., (1993) Science 262:1432 (tomato Pto gene for resistanceto Pseudomonas syringae pv. tomato encodes a protein kinase); Mindrinos,et al., (1994) Cell 78: 1089 (Arabidopsis RSP2 gene for resistance toPseudomonas syringae); McDowell and Woffenden, (2003) Trends Biotechnol.21(4):178-83 and Toyoda, et al., (2002) Transgenic Res. 11(6):567-82. Aplant resistant to a disease is one that is more resistant to a pathogenas compared to the wild type plant.

(B) A gene conferring resistance to fungal pathogens, such as oxalateoxidase or oxalate decarboxylase (Zhou, et al., (1998) Pl. Physiol.117(1):33-41).

(C) A Bacillus thuringiensis (Bt) protein, a derivative thereof or asynthetic polypeptide modeled thereon. See, for example, Geiser, et al.,(1986) Gene 48:109, who disclose the cloning and nucleotide sequence ofa Bf deltaendotoxin gene. Moreover, DNA molecules encodingdelta-endotoxin genes can be purchased from American Type CultureCollection (Manassas, VA), for example, under ATCC Accession Numbers40098, 67136, 31995 and 31998. Other examples of Bacillus thuringiensistransgenes being genetically engineered are given in the followingpatents and patent applications: 5, 188,960; 5,689,052; 5,880,275; WO91/114778; WO 99/31248; WO 01/12731; WO 99/24581; WO 97/40162 and U.S.Application Serial Numbers 10/032,717; 10/414,637; and 10/606,320.

(D) An insect-specific hormone or pheromone such as an ecdysteroid andjuvenile hormone, a variant thereof, a mimetic based thereon, or anantagonist or agonist thereof. See, for example, the disclosure byHammock, et al., (1990) Nature 344:458, of baculovirus expression ofcloned juvenile hormone esterase, an inactivator of juvenile hormone.

(E) An insect-specific peptide which, upon expression, disrupts thephysiology of the affected pest. For example, see the disclosures ofRegan, (1994) J. Biol. Chem. 269:9 (expression cloning yields DNA codingfor insect diuretic hormone receptor) and Pratt, et al., (1989) Biochem.Biophys. Res. Comm. 163:1243 (an allostatin is identified in Diplopterapuntata); Chattopadhyay, et al., (2004) Critical Reviews in Microbiology30(1):33-54 2004; Zjawiony, (2004) J Nat Prod 67(2):300-310; Carlini andGrossi-de-Sa, (2002) Toxicon 40(11):1515- 1539; Ussuf, et al., (2001)Curr Sci. 80(7):84 7-853 and Vasconcelos and Oliveira, (2004) Toxicon44(4):385-403. See also, U.S. Pat. Number 5,266,317 to Tomalski, et al.,who disclose genes encoding insect-specific, paralytic neurotoxins.

(F) An enzyme responsible for a hyperaccumulation of a monterpene, asesquiterpene, a steroid, hydroxamic acid, a phenylpropanoid derivativeor another non-protein molecule with insecticidal activity.

(G) An enzyme involved in the modification, including the posttrans/ationa/ modification, of a biologically active molecule; for example, aglycolytic enzyme, a proteolytic enzyme, a lipolytic enzyme, a nuclease,a cyclase, a transaminase, an esterase, a hydrolase, a phosphatase, akinase, a phosphorylase, a polymerase, an elastase, a chitinase and aglucanase, whether natural or synthetic. See PCT Application Number WO93/02197 in the name of Scott, et al., which discloses the nucleotidesequence of a callase gene. DNA molecules which containchitinase-encoding sequences can be obtained, for example, from the ATCCunder Accession Numbers 39637 and 67152. See also, Kramer, et al.,(1993) Insect Biochem. Molec. Biol. 23:691, who teach the nucleotidesequence of a cDNA encoding tobacco hookworm chitinase, and Kawalleck etal., (1993) Plant Molec. Biol. 21:673, who provide the nucleotidesequence of the parsley ubi4-2 polyubiquitin gene, U.S. Pat. ApplicationSerial Numbers 10/389,432, 10/692,367 and U.S. Pat. Number 6,563,020.

(H) A molecule that stimulates signal transduction. For example, see thedisclosure by Botella, et al., (1994) Plant Molec. Biol. 24:757, ofnucleotide sequences for mung bean calmodulin cDNA clones, and Griess,et al., (1994) Plant Physiol. 104:1467, who provide the nucleotidesequence of a maize calmodulin cDNA clone.

(I) A hydrophobic moment peptide. See, PCT Application Number W095/16776and U.S. Pat. Number 5,580,852 (disclosure of peptide derivatives ofTachyplesin which inhibit fungal plant pathogens) and PCT ApplicationNumber W095/18855 and U.S. Pat. Number 5,607,914 (teaches syntheticantimicrobial peptides that confer disease resistance).

(J) A membrane permease, a channel former or a channel blocker. Forexample, see the disclosure by Jaynes, et al., (1993) Plant Sci. 89:43,of heterologous expression of a cecropin-beta lytic peptide analog torender transgenic tobacco plants resistant to Pseudomonas solanacearum.

(K) A viral-invasive protein or a complex toxin derived therefrom. Forexample, the accumulation of viral coat proteins in transformed plantcells imparts resistance to viral infection and/or disease developmenteffected by the virus from which the coat protein gene is derived, aswell as by related viruses. See Beachy, et al., (1990) Ann. Rev.Phytopathol. 28:451. Coat protein-mediated resistance has been conferredupon transformed plants against alfalfa mosaic virus, cucumber mosaicvirus, tobacco streak virus, potato virus X, potato virus Y, tobaccoetch virus, tobacco rattle virus and tobacco mosaic virus. Id.

(L) An insect-specific antibody or an immunotoxin derived therefrom.Thus, an antibody targeted to a critical metabolic function in theinsect gut would inactivate an affected enzyme, killing the insect. CfTaylor, et al., Abstract #497, SEVENTH INT′L SYMPOSIUM ON MOLECULARPLANT-MICROBE INTERACTIONS (Edinburgh, Scotland, 1994) (enzymaticinactivation in transgenic tobacco via production of single-chainantibody fragments).

(M) A virus-specific antibody. See, for example, Tavladoraki, et al.,(1993) Nature 366:469, who show that transgenic plants expressingrecombinant antibody genes are protected from virus attack.

(N) A developmental-arrestive protein produced in nature by a pathogenor a parasite. Thus, fungal endo alpha-1,4-D-polygalacturonasesfacilitate fungal colonization and plant nutrient release bysolubilizing plant cell wall homo-alpha- 1,4-D-galacturonase. See, Lamb,et al., (1992) Bio/Technology 10: 1436. The cloning and characterizationof a gene which encodes a bean 15 endopolygalacturonase-inhibitingprotein is described by Toubart, et al., (1992) Plant J. 2:367. Adevelopmental-arrestive protein produced in nature by a plant. Forexample, Logemann, et al., (1992) Bio/Technology 10:305, have shown thattransgenic plants expressing the barley ribosome-inactivating gene havean increased resistance to fungal disease.

(O) Genes involved in the Systemic Acquired Resistance (SAR) Responseand/or the pathogenesis related genes. Briggs, (1995) Current Biology5(2):128-131, Pieterse and Van Loon, (2004) Curr. Opin. Plant Bio7(4):456-64 and Somssich, (2003) Ce// 113(7):815-6.

(P) Antifungal genes (Cornelissen and Melchers, (1993) Pl. Physiol.101:709-712 and Parijs, et al., (1991) P/anta 183:258-264 and Bushnell,et al., (1998) Can. J. of Plant Path. 20(2):137-149. Also see, U.S. Pat.Application Number 09/950,933.

(Q) Detoxification genes, such as for fumonisin, beauvericin,moniliformin and zearalenone and their structurally related derivatives.For example, see, U.S. Pat. Number 5,792,931.

(R) Cystatin and cysteine proteinase inhibitors. See, U.S. Pat.Application Serial Number 10/947,979.

(S) Defensin genes. See, W003/000863 and U.S. Pat. Application SerialNumber 10/178,213. (U) Genes that confer resistance to Phytophthora RootRot, such as the Brassica equivalents of the Rps 1, Rps 1-a, Rps 1-b,Rps 1-c, Rps 1-d, Rps 1-e, Rps 1-k, Rps 2, Rps 3-a, Rps 3-b, Rps 3-c,Rps 4, Rps 5, Rps 6, Rps 7 and other Rps genes. See, for example,Shoemaker, et al, (1995) Phytophthora Root Rot Resistance Gene Mappingin Soybean, Plant Genome IV Conference, San Diego, CA.

2. Genes that confer resistance to a herbicide, for example:

(A) A herbicide that inhibits the growing point or meristem, such as animidazalinone or a sulfonylurea. Exemplary genes in this category codefor mutant ALS and AHAS enzyme as described, for example, by Lee, etal., (1988) EMBOJ. 7:1241, and Miki, et al., (1990) Theor. Appl.Genet.80:449, respectively. See also, U.S. Pat. Numbers 5,605,011; 5,013,659;5,141,870; 5,767,361; 5,731,180; 5,304,732; 4,761,373; 5,331,107;5,928,937 and 5,378,824; and international publication WO 96/33270.

(B) Glyphosate (resistance imparted by mutant 5-enolpyruvl-3-phosphikimate synthase (EPSP) and aroA genes, respectively) and otherphosphono compounds such as glufosinate (phosphinothricin acetyltransferase, PAT) and Streptomyces hygroscopicus phosphinothricin-acetyltransferase, bar, genes), and pyridinoxy or phenoxy propionic acids andcycloshexones (ACCase inhibitor-encoding genes). See, for example, U.S.Pat. Number 4,940,835 to Shah, et al., which discloses the nucleotidesequence of a form of EPSP which can confer glyphosate resistance. Seealso, U.S. Pat. Number 7,405,074, and related applications, whichdisclose compositions and means for providing glyphosate resistance.U.S. Pat. Number 5,627,061 to Barry, et al., also describes genesencoding EPSPS enzymes. See also, U.S. Pat. Numbers 6,566,587;6,338,961; 6,248,87681 6,040,497; 5,804,425; 5,633,435; 5,145,783;4,971,908; 5,312,910; 5,188,642; 4,940,835; 5,866,775; 6,225,11481;6,130,366; 5,310,667; 4,535,060; 4,769,061; 5,633,448; 5,510,471; Re.36,449; RE 37,287 E; and 5,491,288; and international publicationsEP1173580; WO 01/66704; EP1173581 and EP1173582. A DNA molecule encodinga mutant aroA gene can be obtained under ATCC Accession Number 39256,and the nucleotide sequence of the mutant gene is disclosed in U.S. Pat.Number 4,769,061 to Comai. European Patent Application Number 0 333 033to Kumada, et al., and U.S. Pat. Number 4,975,374 to Goodman, et al.,disclose nucleotide sequences of glutamine synthetase genes which conferresistance to herbicides such as L- phosphinothricin. The nucleotidesequence of a phosphinothricin-acetyltransferase gene is provided inEuropean Application Number 0 242 246 to Leemans, et al., De Greef, etal., (1989) Bio/Technology 7:61, describe the production of transgenicplants that express chimeric bar genes coding for phosphinothricinacetyl transferase activity. See also, U.S. Pat. Numbers 5,969,213;5,489,520; 5,550,318; 5,874,265; 5,919,675; 5,561,236; 5,648,477;5,646,024; 6,177,616 81 and 5,879,903. Exemplary of genes conferringresistance to phenoxy propionic acids and cycloshexones, such assethoxydim and haloxyfop, are the Acc1-S1, Acc1-S2 and Acc1-S3 genesdescribed by Marshall, et al., (1992) Theor. Appl. Genet. 83:435. Seealso, U.S. Pat. Numbers 5,188,642; 5,352,605; 5,530,196; 5,633,435;5,717,084; 5,728,925; 5,804,425 and Canadian Patent Number 1,313,830.

(C) A herbicide that inhibits photosynthesis, such as a triazine (psbAand gs+ genes) and a benzonitrile (nitrilase gene). Przibilla, et al.,(1991) Plant Cell 3: 169, describe the transformation of Chlamydomonaswith plasmids encoding mutant psbA genes. Nucleotide sequences fornitrilase genes are disclosed in U.S. Pat. Number 4,810,648 to Stalker,and DNA molecules containing these genes are available under ATCCAccession Numbers 53435, 67441 and 67442. Cloning and expression of DNAcoding for a glutathione S-transferase is described by Hayes, et al.,(1992) Biochem. J. 285:173.

(D) Acetohydroxy acid synthase, which has been found to make plants thatexpress this enzyme resistant to multiple types of herbicides, has beenintroduced into a variety of plants (see, e.g., Hattori, et al., (1995)Mo/ Gen Genet 246:419). Other genes that confer tolerance to herbicidesinclude: a gene encoding a chimeric protein of rat cytochrome P4507A1and yeast NADPH- cytochrome P450 oxidoreductase (Shiota, et al., (1994)Plant Physiol 106:17), genes for glutathione reductase and superoxidedismutase (Aono, et al., (1995) Plant Cell Physiol 36: 1687, and genesfor various phosphotransferases (Datta, et al., (1992) Plant Mo/Bio/20:619).

(E) Protoporphyrinogen oxidase (protox) is necessary for the productionof chlorophyll, which is necessary for all plant survival. The protoxenzyme serves as the target for a variety of herbicidal compounds. Theseherbicides also inhibit growth of all the different species of plantspresent, causing their total destruction. The development of plantscontaining altered protox activity which are resistant to theseherbicides are described in U.S. Pat. Numbers 6,288,306 81; 6,282,83781; and 5,767,373; and international publication WO 01/12825.

3. Transgenes that confer or contribute to an altered graincharacteristic, such as:

(A) Altered fatty acids, for example, by

(1) Down-regulation of stearoyl-ACP desaturase to increase stearic acidcontent of the plant. See, Knultzon, et al., (1992) Proc. Natl. Acad.Sci. USA 89:2624 and W099/64579 (Genes for Desaturases to Alter LipidProfiles in Corn),

(2) Elevating oleic acid via FAD-2 gene modification and/or decreasinglinolenic acid via FAD-3 gene modification (see, U.S. Pat. Numbers6,063,947; 6,323,392; 6,372,965 and WO 93/11245),

(3) Altering conjugated linolenic or linoleic acid content, such as inWO 01/12800,

(4) Altering LEC1, AGP, Dek1, Superal1, mi1 ps, various lpa genes suchas Ipa1, lpa3, hpt or hggt. For example, see WO 02/42424, WO 98/22604,WO 03/011015, U.S. Pat. Numbers 6,423,886, 6,197,561, 6,825,397, U.S.Pat. Application Publication Numbers 2003/0079247, 2003/0204870,W002/057439, W003/011015 and Rivera-Madrid, et al., (1995) Proc. Natl.Acad. Sci. 92:5620-5624.

(B) Altered phosphorus content, for example, by the

(1) Introduction of a phytase-encoding gene would enhance breakdown ofphytate, adding more free phosphate to the transformed plant. Forexample, see, Van Hartingsveldt, et al., (1993) Gene 127:87, for adisclosure of the nucleotide sequence of an Aspergil/us niger phytasegene.

(2) Up-regulation of a gene that reduces phytate content. In maize,this, for example, could be accomplished, by cloning and then re-introducing DNA associated with one or more of the alleles, such as theLPA alleles, identified in maize mutants characterized by low levels ofphytic acid, such as in Raboy, et al., (1990) Maydica 35:383 and/or byaltering inositol kinase activity as in WO 02/059324, U.S. Pat.Application Publication Number 2003/0009011, WO 03/027243, U.S. Pat.Application Publication Number 2003/0079247, WO 99/05298, U.S. Pat.Numbers 6,197,561, 6,291,224, 6,391,348, W02002/059324, U.S. Pat.Application Publication Number 2003/0079247, W098/45448, W099/55882,W001/04147.

(C) Altered carbohydrates effected, for example, by altering a gene foran enzyme that affects the branching pattern of starch, a gene alteringthioredoxin. (See, U.S. Pat. Number 6,531,648). See, Shiroza, et al.,(1988) J. Bacterial 170:810 (nucleotide sequence of Streptococcus mutansfructosyltransferase gene), Steinmetz, et al., (1985) Mo/. Gen. Genet.200:220 (nucleotide sequence of Bacillus subtilis levansucrase gene),Pen, et al., (1992) Bio/Technology 10:292 (production of transgenicplants that express Bacillus licheniformis alpha amylase), Elliot, etal., (1993) Plant Molec Biol 21 :515 (nucleotide sequences of tomatoinvertase genes), Sogaard, et al., (1993) J. Biol. Chem. 268:22480 (sitedirected mutagenesis of barley alpha-amylase gene) and Fisher, et al.,(1993) Plant Physiol 102: 1045 (maize endosperm starch branching enzymeII), WO 99/10498 (improved digestibility and/or starch extractionthrough modification of UDP-D-xylose 4-epimerase, Fragile 1 and 2, Ref1,HCHL, C4H), U.S. Pat. Number 6,232,529 (method of producing high oilseed by modification of starch levels (AGP)). The fatty acidmodification genes mentioned above may also be used to affect starchcontent and/or composition through the interrelationship of the starchand oil pathways.

(D) Altered antioxidant content or composition, such as alteration oftocopherol or tocotrienols. For example, see, U.S. Pat. Number6,787,683, U.S. Pat. Application Publication Number 2004/0034886 and WO00/68393 involving the manipulation of antioxidant levels throughalteration of a phytl prenyl transferase (ppt), WO 03/082899 throughalteration of a homogentisate geranyl geranyl transferase (hggt).

(E) Altered essential seed amino acids. For example, see, U.S. Pat.Number 6,127,600 (method of increasing accumulation of essential aminoacids in seeds), U.S. Pat. Number 6,080,913 (binary methods ofincreasing accumulation of essential amino acids in seeds), U.S. Pat.Number 5,990,389 (high lysine), WO99/40209 (alteration of amino acidcompositions in seeds), W099/29882 (methods for altering amino acidcontent of proteins), U.S. Pat. Number 5,850,016 (alteration of aminoacid compositions in seeds), W098/20133 (proteins with enhanced levelsof essential amino acids), U.S. Pat. Number 5,885,802 (high methionine),U.S. Pat. Number 5,885,801 (high threonine), U.S. Pat. Number 6,664,445(plant amino acid biosynthetic enzymes), U.S. Pat. Number 6,459,019(increased lysine and threonine), U.S. Pat. Number 6,441,274 (planttryptophan synthase beta subunit), U.S. Pat. Number 6,346,403(methionine metabolic enzymes), U.S. Pat. Number 5,939,599 (highsulfur), U.S. Pat. Number 5,912,414 (increased methionine), W098/56935(plant amino acid biosynthetic enzymes), W098/45458 (engineered seedprotein having higher percentage of essential amino acids), W098/42831(increased lysine), U.S. Pat. Number 5,633,436 (increasing sulfur aminoacid content), U.S. Pat. Number 5,559,223 (synthetic storage proteinswith defined structure containing programmable levels of essential aminoacids for improvement of the nutritional value of plants), W096/01905(increased threonine), W095/15392 (increased lysine), U.S. Pat.Application Publication Number 2003/0163838, U.S. Pat. ApplicationPublication Number 2003/0150014, U.S. Pat. Application PublicationNumber 2004/0068767, U.S. Pat. Number 6,803,498, W001/79516, andW000/09706 (Ces A: cellulose synthase), U.S. Pat. Number 6,194,638(hemicellulose), U.S. Pat. Number 6,399,859 and U.S. Pat. ApplicationPublication Number 2004/0025203 (UDPGdH), U.S. Pat. Number 6,194,638(RGP).

4. Genes that control pollination, hybrid seed production, ormale-sterility: There are several methods of conferring genetic malesterility available, such as multiple mutant genes at separate locationswithin the genome that confer male sterility, as disclosed in U.S. Pat.Numbers 4,654,465 and 4,727,219 to Brar, et al., and chromosomaltranslocations as described by Patterson in U.S. Pat. Numbers 3,861,709and 3,710,511. In addition to these methods, Albertsen, et al., U.S.Pat. Number 5,432,068, describe a system of nuclear male sterility whichincludes: identifying a gene which is critical to male fertility;silencing this native gene which is critical to male fertility; removingthe native promoter from the essential male fertility gene and replacingit with an inducible promoter; inserting this genetically engineeredgene back into the plant; and thus creating a plant that is male sterilebecause the inducible promoter is not “on” resulting in the malefertility gene not being transcribed. Fertility is restored by inducing,or turning “on”, the promoter, which in turn allows the gene thatconfers male fertility to be transcribed.

(A) Introduction of a deacetylase gene under the control of atapetumspecific promoter and with the application of the chemicalN-Ac-PPT (WO01/29237).

(B) Introduction of various stamen-specific promoters (WO 92/13956, WO92/13957).

(C) Introduction of the barnase and the barstar gene (Paul, et al.,(1992) Plant Mo/. Biol. 19:611-622). For additional examples of nuclearmale and female sterility systems and genes, see also, U.S. Pat. Numbers5,859,341; 6,297,426; 5,478,369; 5,824,524; 5,850,014 and 6,265,640.Also see, U.S. Pat. Number 5,426,041 (invention relating to a method forthe preparation of a seed of a plant comprising crossing a male sterileplant and a second plant which is male fertile), U.S. Pat. Number6,013,859 (molecular methods of hybrid seed production) and U.S. Pat.Number 6,037,523 (use of male tissue-preferred regulatory region inmediating fertility).

5. Genes that create a site for site specific DNA integration. Thisincludes the introduction of FRT sites that may be used in the FLP/FRTsystem and/or Lox sites that may be used in the Cre/Loxp system. Forexample, see, Lyznik, et al., (2003) “Site-Specific Recombination forGenetic Engineering in Plants”, Plant Cell Rep 21 :925-932 and WO99/25821. Other systems that may be used include the Gin recombinase ofphage Mu (Maeser, et al., 1991), the Pin recombinase of E. coli(Enomoto, et al., 1983), and the R/RS system of the pSR 1 plasmid(Araki, et al., 1992).

6. Genes that affect abiotic stress resistance (including but notlimited to flowering, ear and seed development, enhancement of nitrogenutilization efficiency, altered nitrogen responsiveness, droughtresistance or tolerance, cold resistance or tolerance, and saltresistance or tolerance) and increased yield under stress. For example,see, WO 00/73475 where water use efficiency is altered throughalteration of malate; U.S. Pat. Numbers 5,892,009, 5,965,705, 5,929,305,5,891,859, 6,417,428, 6,664,446, 6,706,866, 6,717,034, 6,801,104,W02000060089, W02001026459, W02001035725, W02001034726, W02001035727,W02002015675, W02003013227, W02001036444, W02002017430, W02003013228,W02001036597, W02002077185, W02003014327, W02001036598, W02002079403,W02004031349, W02004076638, W09809521 and W09938977 describing genes,including CBF genes and transcription factors effective in mitigatingthe negative effects of freezing, high salinity, and drought on plants,as well as conferring other positive effects on plant phenotype; U.S.Pat. Application Publication Number 2004/0148654 and W001/36596 whereabscisic acid is altered in plants resulting in improved plant phenotypesuch as increased yield and/or increased tolerance to abiotic stress;W02000/006341, W004/090143, U.S. Pat. Application Serial Numbers10/817483 and 09/545,334 where cytokinin expression is modifiedresulting in plants with increased stress tolerance, such as droughttolerance, and/or increased yield. Also see W00202776, W003052063,JP2002281975, U.S. Pat. Number 6,084,153, W00164898, U.S. Pat. Number6,177,275 and U.S. Pat. Number 6,107,547 (enhancement of nitrogenutilization and altered nitrogen responsiveness). For ethylenealteration, see, U.S. Pat. Application Publication Numbers 2004/0128719,2003/0166197 and W0200032761. For plant transcription factors ortranscriptional regulators of abiotic stress, see e.g., U.S. Pat.tApplication Publication Number 2004/0098764 or U.S. Pat. ApplicationPublication Number 2004/0078852. Other genes and transcription factorsthat affect plant growth and agronomic traits such as yield, flowering,plant growth and/or plant structure, can be introduced or introgressedinto plants, see, e.g., W097/49811 (LHY), W098/56918 (ESD4), W097/10339and US6573430 (TFL), US6713663 (FT), W096/14414 (CON), W096/38560,W001/21822 (VRN1), W000/44918 (VRN2), W099/49064 (GI), W000/46358 (FRI),W097/29123, U.S. Pat. Numbers 6,794,560, 6,307,126 (GAi), W099/09174 (08and Rht), and W02004076638 and W02004031349 (transcription factors).

Seed Cleaning

This invention is also directed to methods for producing cleaned canolaseed by cleaning seed of variety 9CN0089. “Cleaning a seed” or “seedcleaning” refers to the removal of foreign material from the surface ofthe seed. Foreign material to be removed from the surface of the seedincludes but is not limited to fungi, bacteria, insect material,including insect eggs, larvae, and parts thereof, and any other peststhat exist on the surface of the seed. The terms “cleaning a seed” or“seed cleaning” also refer to the removal of any debris or low quality,infested, or infected seeds and seeds of different species that areforeign to the sample.

This invention is also directed to produce subsequent generations ofseed from seed of variety 9CN0089, harvesting the subsequent generationof seed; and planting the subsequent generation of seed.

Seed Treatment “Treating a seed” or “applying a treatment to a seed”refers to the application of a composition to a seed as a coating orotherwise. The composition may be applied to the seed in a seedtreatment at any time from harvesting of the seed to sowing of the seed.The composition may be applied using methods including but not limitedto mixing in a container, mechanical application, tumbling, spraying,misting, and immersion. Thus, the composition may be applied as aslurry, a mist, or a soak. The composition to be used as a seedtreatment can be a pesticide, fungicide, insecticide, or antimicrobial.For a general discussion of techniques used to apply fungicides toseeds, see “Seed Treatment,” 2d ed., (1986), edited by K. A Jeffs(chapter 9). Industrial Applicability The seed of the 9CN0089 variety,the plant produced from such seed, various parts of the 9CN0089 hybridcanola plant or its progeny, a canola plant produced from the crossingof the 9CN0089 variety, and the resulting seed, can be utilized in theproduction of an edible vegetable oil or other food products inaccordance with known techniques. The remaining solid meal componentderived from seeds can be used as a nutritious livestock feed.

DEPOSITS

Applicant(s) have made a deposit of at least 2500 seeds of 9CN0089Canola hybrid variety with NCIMB Ltd, Ferguson Building, CraibstoneEstate, Bucksburn, Aberdeen, AB21 9YA Scotland. The seeds of 9CN0089Canola hybrid variety were deposited on [XXXXXX] with NCIMB underAccession Number [XXXXXX]. The seeds were taken from the seed stockmaintained by BASF Canada Inc. prior to the filing date of thisapplication. Access to these deposits will be available during thependency of the application to the Commissioner of Patents andTrademarks and persons determined by the Commissioner to be entitledthereto upon request. Upon allowance of any claims in the application,the Applicant will make available to the public, pursuant to 37 C.F.R.1.808, sample(s) of the deposit of at least 2500 seeds of 9CN0089 Canolahybrid variety all which are with NCIMB Ltd, Ferguson Building,Craibstone Estate, Bucksburn, Aberdeen, AB21 9YA Scotland. Thesedeposits of seed of 9CN0089 Canola hybrid variety will be maintained inthe NCIMB depository, which is a public depository, for a period of 30years, or 5 years after the most recent request, or for the enforceablelife of the patent, whichever is longer, and will be replaced if itbecomes nonviable during that period. Additionally, Applicant hassatisfied all the requirements of NCIMB, including providing anindication of the viability of the sample upon deposit. Applicant has noauthority to waive any restrictions imposed by law on the transfer ofbiological material or its transportation in commerce. Applicant(s) donot waive any infringement of their rights granted under this patent orrights applicable to canola hybrid 9CN0089 or of the parental Canolavarieties under the Plant Breeders’ Rights Act (S.C. 1990, c.20).

EXAMPLES

The invention is illustrated by the following examples. However, theinvention is not limited to the examples.

WCC/RCC is the abbreviation for the Western Canadian Canola RapeseedRecommending Committee.

Example 1 Summary of Variety Characteristics

9CN0089 is a mid to late-maturing canola hybrid (1.6 days later than theaverage of checks) which is suitable for most growing zones in Canada,this hybrid provides high yield and is best fitted to the longer growingregions. 9CN0089 is reduced in pod shatter allowing both straightcutting or delayed swathing for harvest. The pollination control used in9CN0089 is the nuclear genetic male sterility system based onbarstar/barnase (SeedLink TM, BASF). 9CN0089 is a hybrid that is averagein height and provides adequate lodging resistance. In addition to beingrated as blackleg-resistant, 9CN0089 also possesses clubroot resistanceto predominant clubroot pathotypes identified in Canada in 2017.

9CN0089 has a black seed coat color. The oil content is 46.3% on a wholedry seed basis. The protein content is 45.8% on a whole dry seed basis.Glucosinolates content of 9CN0089 is 10.9 umol per gram of whole seed ata moisture of 8.5 %, therefore low.

Petiole length, leaf width, and silique length may be considered asfurther variety distinguishing characteristics. 9CN0089 is tolerant tothe herbicide glufosinate and salts thereof, e.g. glufosinate ammonium.

TABLE 1 Trait Code Trait Mean of 9CN0089 Description of 9CN0089 Mean ofPA7CN138 Mean of PR0CN783 1.1 Botanical name Brassica napus L. 1.2Season type Spring 1.3 CSGA recognized type of variety Hybrid 1.4Pollination control Nuclear genetic male sterility (Seedlink™) 2.3 Stemanthocyan intensity (1=absent or very weak, 3=weak, 5=medium, 7=strong,9=very strong) 1 1 1 2.4 Leaf type (1=petiolate, 9=lyrate) 1 1 1 2.6Leaf length (3=short, 5=medium, 7=long) 9 5 5 2.7 Leaf width (3=narrow,5=medium, 7= wide) 7 4 4 2.8 Leaf colour at 5-leaf stage (1=light green,2= medium green, 3=dark green, 4=blue-green) 2 3 2 2.12 Leaf lobedevelopment (observe fully developed upper stem leaves): (1=absent orvery weak, 3=weak, 5=medium, 7=strong, 9=very strong) 9 8 7 2.13 Numberof leaf lobes 5.6 4.4 4.0 2.14 Petiole length (lobed varieties only)(3=short, 5=medium, 7=long) 1 1 9 2.15 Leaf margin shape (1=undulating,2=rounded, 3=sharp) 3 3 3 2.16 Leaf margin indentation (1 =absent orvery weak (very shallow), 3=weak (shallow), 5=medium, 7=strong (deep),9=very strong (very deep) 5 5 7 2.17 Leaf attachment to stem (1=completeclasping, 2=partial clasping, 3=non-clasping) 2 2 2 3.1 Time toflowering (number of days from planting to 50 % of plants showing one ormore open flowers) 43 42 45 3.2 Plant height at maturity (3=short,5=medium, 7=tall) 7 5 7 3.4 Flower bud location (1=buds above mostrecently opened flowers, 9=buds below most recently opened flowers) 1 11 3.5 Petal colour (observe on frist day of flowering) (1=white, 2=lightyellow, 3=medium yellow, 4=dark yellow, 5=orange, 6=other) 3 3 3 3.6Petal length (3=short, 5=medium, 7=long) 5 5 5 3.7 Petal width(3=narrow, 5=medium, 7=wide) 5 5 5 3.10 Anther fertility (measured bypollen production) (1=sterile, 9= all anthers shedding pollen) 1 9 3.11Pod (silique) length (1=short (< 7 cm), 5=medium (7 to 10 cm), 9=long (>10 cm) 5 5 3 3.13 Pod (silique) angle (1=erect, 3=semi-erect,5=horizontal, 7=slightly drooping, 9=drooping) 9 9 9 3.14 Pod (silique)beak length (3=short, 5=medium, 7=long) 3 3 1 3.15 Pedicel length(3=short, 5=medium, 7=long) 3 3 3 3.17 Time to maturity (number of daysfrom planting to physiological maturity) 95 93.5 98 4.1 Seed coat colour(1=black, 2=brown, 3 =tan, 4=yellow, 5=mixed, 6=other) 1 1 1 5.1 ShatterResistance (1=not tested, 3=poor, 5=fair, 7=good, 9= does not shatter) 77 7 5.2 Lodging resistance (1=not tested, 3=poor, 5=fair, 7=good,9=excellent) 7 6 8 6.2 Blackleg (Leptospheria maculans/Phoma ligam)(1=resistant, 3 moderately resistant, 5=moderately susceptible,7=susceptible, 9=highly susceptible) 1 1 1 6.3 Club Root (Plasmodiophorabrassicae Woronin) (1=resistant, 3 moderately resistant, 5=moderatelysusceptible, 7=susceptible, 9=highly susceptible) 1 1 5 7.1.1 Resistanceto herbicides Glufosinate ammonium 8.1 Oil content (percentage, wholedry seed basis) 47 45.3 48.3 8.2.3 Oleic acid (C18:1) as percentage oftotal fatty acids in seed oil 0 0 0 8.2.5 Linolenic acid (C18:3) aspercentage of total fatty acids in seed oil 9.2 8.5 9.1 8.2.6 Erucicacid (C22: 1) as percentage of total fatty acids in seed oil 0 0 0 8.2.7Total saturated fats content as percentage of total fatty acids in seedoil 6.8 7.8 6.1 8.5 Protein content (percentage, whole oil-free dry seedbasis) 45.1 45.2 45.3 8.6 Glucosinolates content (µmol of totalglucosinolates per gram whole seed, 8.5 % moisture basis) (1=very low (<10 µmol per gram, 2=low (10-15 µmol per gram), 3=medium (15-20 µmol pergram), 4=high (>20 µmol per gram) 11 15 8.7

Example 2

9CN0089 was tested in 2019 and 2020 trials following WCC/RCC guidelines.WCC/RRC guidelines were followed for analyzing quality parameters. Yieldand agronomic traits were recorded and seed samples were collected andwere analyzed for quality traits such as oil and protein percent, totalwhole seed glucosinolates, and eruic acid content. Protein and oilcontent was according to WCC/RRC criteria. One station represents onetrial at a certain location in a specific year.

Yield is expressed as percentage of the yield of the standard of theCanola hybrids 45H33 (Pioneer Hi-Bred) and L233P (BASF Canada Inc.).Oil, protein and saturate fatty acid content were according to WCC/RCCcriteria. Maturity allows wide adaptation across all zones.Glucosinolates content was 10.9 umoles/g whole seed at 8.5% moisture(all zones). Erucic acid content was 0.00% (all zones).

TABLE 2 Yield (% of 45H33 & L233P) Overall Short season zone Mid seasonzone Long season zone Yield in Trials 2019 and 2020 109.8 106.9 106.0118.9 Number of Station Years 31 9 13 9

Example 3 Blackleg Resistance

Blackleg resistance is rated on a scale of 0 to 5: a plant with zerorating is completely immune to disease while a plant with ″5″s rating isdead due to blackleg infection. Plants in blackleg trials are rated atthe 5.2 stage on the Harper and Berkenkamp scale and that evaluation ofdisease reaction is based on the extent of the infection throughout thestem. This was evaluated by cutting open the stem at the site of thecanker.

Tests were rated using a 0-5 scale, as follows:

-   0 - no diseased tissue visible in the cross-section-   1 - Diseased tissue occupies up to 25% of cross-section-   2 - Diseased tissue occupies 26-50% of cross-section-   3 - Diseased tissue occupies 51-75% of cross-section-   4 - Diseased tissue occupies more than 75% of cross-section with    little or no constriction of affected tissues-   5 - Diseased tissue occupies 100% of cross-section with significant    constriction of affected tissues; tissue dry and brittle; plant dead

Canola variety “Westar” is included as an entry/control in each blacklegtrial. Tests are considered valid when the mean rating for Westar isgreater than or equal to 2.6 and less than or equal to 4.5. (In yearswhen there is poor disease development in Western Canada the WCC/RRC mayaccept the use of data from trials with a rating for Westar exceeding2.0.). 9CN0089 has an “R” rating for Blackleg (23.8% of Westar)according to the 2019 and 2020 trials described in example 2.

Example 4 Shatter Resistance Evaluation Trial

9CN0089 was compared to the Canola hybrid L230 (BASF Canada Inc.) whichis moderately susceptible to pod shatter. Shatter resistance wasassessed at harvest on a scale from 1 = all pods intact to 5 = severeshatter loss. Trials were evaluated at four different locationsthroughout Western Canada in 2020. Location 1 had no measurable podshattering - thus all ratings as a 1.00.

TABLE 3 Hybrid Location 1 Location 2 Location 3 Location 4 Overall %Check L230 (check) 4.00 4.00 3.00 1.50 3.13 52% 9CN0089 2.00 2.00 1.001.50 1.63 100%

9CN0089 was found to be 48% more pod shatter tolerant than the checkL230.

1-88. (canceled)
 89. A plant or part thereof of a Canola hybrid varietydesignated 9CN0089, wherein a representative sample of seed of thatvariety has been deposited under Accession Number [XXXXXX], wherein thepart thereof comprises at least one cell of Canola hybrid varietydesignated 9CN0089.
 90. A method for the protection of a group ofcultivated plants of the plant of claim 89, comprising one or more of:(a) applying a composition comprising one or more herbicidal activeingredients, in a field wherein weeds are to be controlled; (b) applyinga composition comprising one or more fungicidal active ingredients, in afield wherein harmful microorganisms are to be controlled; and/or (c)applying a composition comprising one or more insecticidal activeingredients, in a field wherein pests are to be controlled.
 91. Themethod according to claim 90, wherein: (a) the one or more herbicidalactive ingredients is amitrol, carfentrazone, clethodim, clopyralid,dicamba, diquat, ethalfluralin, ethametsulfuron-methyl, florasulam,imazamox, imazapyr, glufosinate, glufosinate-ammonium, glyphosate, MCPAamine, MCPA ester, metsulfuron, quizalofop-p-ethyl, quinclorac,saflufenacil, triallate, and/or trifluralin; (b) the one or morefungicidal active ingredients is azoxystrobin, benzovindiflupyr,boscalid, cyprodinil, fludioxonil, fluxapyroxad, fluopyram,ipfentrifluconazole, iprodione, isoflucypram, metalaxyl, mefenoxam,mefentrifluconazole, metconazole, penthiopyrad, picoxystrobin,propiconazole, prothioconazole, pyraclostrobin, pyraziflumid,pydiflumetofen, sedaxane, and/or tebuconazole; and/or (c) the one ormore insecticidal active ingredients is broflanilide, carbaryl,carbofuran, chlorantraniliprole, chlorpyrifos, cypermethrin,cyclaniliprole, cyhalodiamide, clothianidin, deltamethrin, dimethoate,cyantraniliprole, cyhalothrin-lambda, imidacloprid, lambda-cyhalothrin,permethrin, sulfoxaflor, spirotetramate, tetraniliprole, and/orthiamethoxam.
 92. The method according to claim 91, wherein the one ormore herbicidal active ingredients comprises glufosinate or glufosinateammonium.
 93. A method of producing an inbred plant, the methodcomprising selecting a plant and selfing the selected plant and itsdescendants for several generations to produce the inbred plant, whereinthe selected plant is derived from the plant of claim
 89. 94. The methodof claim 93, further comprising doubling a haploid to produce a doublehaploid inbred plant, wherein the haploid is the selected plant ordescendant thereof derived from the Canola plant of Canola hybridvariety 9CN0089.
 95. A method of producing F2 seed, the methodcomprising selfing the Canola hybrid plant of claim 89, or breeding saidplant with another plant to produce F1 seed, growing said F1 seed toproduce F1 plants, and selfing or breeding said F1 plants to produce F2seed.
 96. A method of producing a clean seed, the method comprisingobtaining the seed of Canola hybrid variety 9CN0089, wherein arepresentative sample of seed of that variety has been deposited underAccession Number [XXXXXX], and cleaning said seed.
 97. A treated seed ofCanola hybrid variety 9CN0089, representative seed of said varietyhaving been deposited under the Accession Number [XXXXXX].
 98. A methodof producing a treated seed of claim 97, the method comprising obtainingthe seed of Canola hybrid variety 9CN0089, wherein a representativesample of seed of that variety has been deposited under Accession Number[XXXXXX], and treating said seed.
 99. A method of producing a commodityproduct, the method comprising obtaining seed produced by an F1 hybridCanola plant designated 9CN0089, seed of said hybrid having beendeposited under Accession Number [XXXXXX], and preparing the commodityproduct, wherein said commodity product comprises seed oil, meal, fiber,or protein.
 100. A method of producing a commercial crop, the methodcomprising planting the seed of Canola hybrid variety 9CN0089, wherein arepresentative sample of seed of that variety has been deposited underAccession Number [XXXXXX], and growing the commercial crop.